Synthesis of cyclic dipurinic acids as potential inhibitors targeting diguanylate cyclases

CHING SHI MIN

Publication

Synthesis of cyclic dipurinic acids as potential inhibitors targeting diguanylate cyclases

CHING SHI MIN

Abstract

Five analogues with the c-di-GMP backbone structure but with varied bases, including c-di-GMP itself were synthesized with reasonable yield of 10-15% after 8 steps. They were designed as antimicrobial agents to inhibit biofilm formation, which is responsible for diseases such as chronic cystic fibrosis and nosocomial bacteremia. These compounds were subsequently tested for biological activities on Slr 1143, diguanylate cyclase of Synechocystis sp., that was overexpressed from the recombinant plasmid which contained the gene of interest and subsequently, purified by affinity chromatography.