PCR-RFLP genotyping for exon 1 and promoter region mutations of the human Mannose Binding Lectin (MBL-2) gene

Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jim.2005.06.001

Title:	PCR-RFLP genotyping for exon 1 and promoter region mutations of the human Mannose Binding Lectin (MBL-2) gene
Authors:	Tin, S.K. Thumboo, J. Fong, K.Y. Koh, D.R. Lee, L.Y.
Keywords:	Genotype Haplotype Mannose Binding Lectin MBL PCR-RFLP
Issue Date:	2005
Citation:	Tin, S.K., Thumboo, J., Fong, K.Y., Koh, D.R., Lee, L.Y. (2005). PCR-RFLP genotyping for exon 1 and promoter region mutations of the human Mannose Binding Lectin (MBL-2) gene. Journal of Immunological Methods 303 (1-2) : 148-151. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jim.2005.06.001
Abstract:	The Mannose Binding Lectin (MBL) plays an important role in innate immunity and its genetic deficiencies are associated with frequent and prolonged infections. Serum MBL determination may not accurately detect acute phase protein levels and it is also difficult to detect dysfunctional protein. Genotyping of the exon 1 and promoter regions in the MBL gene will provide useful information on the presence of deficiencies in patients. A reproducible PCR-RFLP method is described to accurately detect genotypes of exon 1 and polymorphic haplotypes of the promoter region in the MBL gene. © 2005 Elsevier B.V. All rights reserved.
Source Title:	Journal of Immunological Methods
URI:	http://scholarbank.nus.edu.sg/handle/10635/27084
ISSN:	00221759
DOI:	10.1016/j.jim.2005.06.001
Appears in Collections:	Staff Publications

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