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Title: PDADMAC/Alginate-Coated Gold Nanorod For Eradication of Staphylococcus Aureus Biofilms
Authors: Manimaran, M
Teo, YY
Kah, JCY 
Beishenaliev, A
Loke, YL
Foo, YY
Ng, SF
Chee, CF
Chin, SP
Faruqu, FN
Chang, CY
Misran, M
Chung, LY
Leo, BF
Chiou, SH
Chang, CC
Tay, ST
Kiew, LV
Keywords: MRSA
S. aureus
gold nanorod
Quaternary Ammonium Compounds
Staphylococcus aureus
Anti-Bacterial Agents
Staphylococcal Infections
Methicillin-Resistant Staphylococcus aureus
Cell Line
Microbial Sensitivity Tests
Metal Nanoparticles
Issue Date: 1-Jan-2024
Publisher: Informa UK Limited
Citation: Manimaran, M, Teo, YY, Kah, JCY, Beishenaliev, A, Loke, YL, Foo, YY, Ng, SF, Chee, CF, Chin, SP, Faruqu, FN, Chang, CY, Misran, M, Chung, LY, Leo, BF, Chiou, SH, Chang, CC, Tay, ST, Kiew, LV (2024-01-01). PDADMAC/Alginate-Coated Gold Nanorod For Eradication of Staphylococcus Aureus Biofilms. International Journal of Nanomedicine 19 : 3697-3714. ScholarBank@NUS Repository.
Abstract: Introduction: Over 75% of clinical microbiological infections are caused by bacterial biofilms that grow on wounds or implantable medical devices. This work describes the development of a new poly(diallyldimethylammonium chloride) (PDADMAC)/alginate-coated gold nanorod (GNR/Alg/PDADMAC) that effectively disintegrates the biofilms of Staphylococcus aureus (S. aureus), a prominent pathogen responsible for hospital-acquired infections. Methods: GNR was synthesised via seed-mediated growth method, and the resulting nanoparticles were coated first with Alg and then PDADMAC. FTIR, zeta potential, transmission electron microscopy, and UV–Vis spectrophotometry analysis were performed to characterise the nanoparticles. The efficacy and speed of the non-coated GNR and GNR/Alg/PDADMAC in disintegrating S. aureus-preformed biofilms, as well as their in vitro biocompatibility (L929 murine fibroblast) were then studied. Results: The synthesised GNR/Alg/PDADMAC (mean length: 55.71 ± 1.15 nm, mean width: 23.70 ± 1.13 nm, aspect ratio: 2.35) was biocompatible and potent in eradicating preformed biofilms of methicillin-resistant (MRSA) and methicillin-susceptible S. aureus (MSSA) when compared to triclosan, an antiseptic used for disinfecting S. aureus colonisation on abiotic surfaces in the hospital. The minimum biofilm eradication concentrations of GNR/Alg/PDADMAC (MBEC50 for MRSA biofilm = 0.029 nM; MBEC50 for MSSA biofilm = 0.032 nM) were significantly lower than those of triclosan (MBEC50 for MRSA biofilm = 10,784 nM; MBEC50 for MRSA biofilm 5967 nM). Moreover, GNR/Alg/PDADMAC was effective in eradicating 50% of MRSA and MSSA biofilms within 17 min when used at a low concentration (0.15 nM), similar to triclosan at a much higher concentration (50 µM). Disintegration of MRSA and MSSA biofilms was confirmed by field emission scanning electron microscopy and confocal laser scanning microscopy. Conclusion: These findings support the potential application of GNR/Alg/PDADMAC as an alternative agent to conventional antiseptics and antibiotics for the eradication of medically important MRSA and MSSA biofilms.
Source Title: International Journal of Nanomedicine
ISSN: 1176-9114
DOI: 10.2147/IJN.S452085
Appears in Collections:Staff Publications

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