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Title: An efficient and simple method for enriching metaphase cells for dicentric chromosome assay
Authors: Ryo Nakayama
Thanh-Mai Tran
Donovan Anderson
Valerie Swee Ting Goh 
Yohei Fujishima
Tomisato Miura
Issue Date: 4-Mar-2024
Publisher: Oxford University Press
Citation: Ryo Nakayama, Thanh-Mai Tran, Donovan Anderson, Valerie Swee Ting Goh, Yohei Fujishima, Tomisato Miura (2024-03-04). An efficient and simple method for enriching metaphase cells for dicentric chromosome assay. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial 4.0 International
Abstract: As compared to peripheral blood mononuclear cell (PBMC) culture, lower mitotic index is seen in whole blood (WB) culture, but WB can be directly used for culture in dicentric chromosome assay (DCA). The purpose of this study is to develop a simple protocol for metaphase enrichment to improve the metaphase frequency of WB culture. Fixed cells were obtained after performing WB and PBMC cultures for DCA after conventional fixation. An additional low-speed centrifugation of 200 × g for 1 min was performed, separating the fixed cells of WB culture into a pellet and a supernatant fraction. The additional low-speed centrifugation enriched metaphase frequency and provided a mitotic index comparable to the PBMC culture in the pellet fraction. Our study suggests that it is possible to increase the number of metaphase cells on slides using the slow centrifugation method, which could contribute to the efficiency of chromosome aberration analysis in biodosimetry.
ISSN: 1742-3406
DOI: 10.1093/rpd/ncae048
Rights: Attribution-NonCommercial 4.0 International
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