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Title: Optimizing chemical-induced premature chromosome condensation assay for rapid estimation of high-radiation doses
Authors: Ryo Nakayama
Donovan Anderson
Valerie Swee Ting Goh 
Yohei Fujishima
Kaito Yanagidate
Kentaro Ariyoshi
Kosuke Kasai
Mitsuaki A. Yoshida
William F. Blakely
Tomisato Miura
Issue Date: 19-Jan-2024
Citation: Ryo Nakayama, Donovan Anderson, Valerie Swee Ting Goh, Yohei Fujishima, Kaito Yanagidate, Kentaro Ariyoshi, Kosuke Kasai, Mitsuaki A. Yoshida, William F. Blakely, Tomisato Miura (2024-01-19). Optimizing chemical-induced premature chromosome condensation assay for rapid estimation of high-radiation doses. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Series/Report no.: Radiation Protection Dosimetry;
Abstract: In the event of exposure to high doses of radiation, prompt dose estimation is crucial for selecting appropriate treatment modalities, such as cytokine therapy or stem cell transplantation. The chemical-induced premature chromosome condensation (PCC) method offers a simple approach for such dose estimation with significant radiation exposure, but its 48-h incubation time poses challenges for early dose assessment. In this study, we optimized the chemical-induced PCC assay for more rapid dose assessment. A sufficient number of PCC and G2/M-PCC cells were obtained after 40 h of culture for irradiated human peripheral blood up to 20 Gy. By adding caffeine (final concentration of 1 mM) at 34 h from the start of culture, G2/M-PCC index increased by 1.4-fold in 10 Gy cultures. There was also no significant difference in the G2/M-PCC ring frequency induced for doses 0 to 15 Gy between our 40-h caffeine-supplemented chemical-induced PCC method and the conventional 48-h PCC assay.
ISSN: 0144-8420
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Appears in Collections:Staff Publications

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