Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41592-023-01822-1
Title: High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
Authors: Bevilacqua, C
Gomez, JM
Fiuza, UM
Chan, CJ 
Wang, L
Hambura, S
Eguren, M
Ellenberg, J
Diz-Muñoz, A
Leptin, M
Prevedel, R
Keywords: Animals
Mice
Microscopy
Embryonic Development
Drosophila
Embryo, Nonmammalian
Imaging, Three-Dimensional
Issue Date: 30-Mar-2023
Publisher: Springer Science and Business Media LLC
Citation: Bevilacqua, C, Gomez, JM, Fiuza, UM, Chan, CJ, Wang, L, Hambura, S, Eguren, M, Ellenberg, J, Diz-Muñoz, A, Leptin, M, Prevedel, R (2023-03-30). High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development. Nature Methods 20 (5) : 755-760. ScholarBank@NUS Repository. https://doi.org/10.1038/s41592-023-01822-1
Abstract: Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos.
Source Title: Nature Methods
URI: https://scholarbank.nus.edu.sg/handle/10635/241632
ISSN: 1548-7091
1548-7105
DOI: 10.1038/s41592-023-01822-1
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