Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41592-023-01822-1
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dc.titleHigh-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
dc.contributor.authorBevilacqua, C
dc.contributor.authorGomez, JM
dc.contributor.authorFiuza, UM
dc.contributor.authorChan, CJ
dc.contributor.authorWang, L
dc.contributor.authorHambura, S
dc.contributor.authorEguren, M
dc.contributor.authorEllenberg, J
dc.contributor.authorDiz-Muñoz, A
dc.contributor.authorLeptin, M
dc.contributor.authorPrevedel, R
dc.date.accessioned2023-06-07T03:51:50Z
dc.date.available2023-06-07T03:51:50Z
dc.date.issued2023-03-30
dc.identifier.citationBevilacqua, C, Gomez, JM, Fiuza, UM, Chan, CJ, Wang, L, Hambura, S, Eguren, M, Ellenberg, J, Diz-Muñoz, A, Leptin, M, Prevedel, R (2023-03-30). High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development. Nature Methods 20 (5) : 755-760. ScholarBank@NUS Repository. https://doi.org/10.1038/s41592-023-01822-1
dc.identifier.issn1548-7091
dc.identifier.issn1548-7105
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/241632
dc.description.abstractBrillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos.
dc.publisherSpringer Science and Business Media LLC
dc.sourceElements
dc.subjectAnimals
dc.subjectMice
dc.subjectMicroscopy
dc.subjectEmbryonic Development
dc.subjectDrosophila
dc.subjectEmbryo, Nonmammalian
dc.subjectImaging, Three-Dimensional
dc.typeArticle
dc.date.updated2023-06-05T23:46:55Z
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1038/s41592-023-01822-1
dc.description.sourcetitleNature Methods
dc.description.volume20
dc.description.issue5
dc.description.page755-760
dc.published.statePublished
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