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https://doi.org/10.1038/s41598-022-19471-3
Title: | Reconstituted TAD-size chromatin fibers feature heterogeneous nucleosome clusters | Authors: | Korolev, Nikolay Zinchenko, Anatoly Soman, Aghil Chen, Qinming Wong, Sook Yi Berezhnoy, Nikolay Basak, Rajib van der Maarel, Johan RC van Noort, John Nordenskiold, Lars |
Keywords: | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics MOLECULE FORCE SPECTROSCOPY INDIVIDUAL NUCLEOSOMES LINKER HISTONE DNA COMPACTION DYNAMICS REVEALS ORGANIZATION SEQUENCE CORE |
Issue Date: | 16-Sep-2022 | Publisher: | NATURE PORTFOLIO | Citation: | Korolev, Nikolay, Zinchenko, Anatoly, Soman, Aghil, Chen, Qinming, Wong, Sook Yi, Berezhnoy, Nikolay, Basak, Rajib, van der Maarel, Johan RC, van Noort, John, Nordenskiold, Lars (2022-09-16). Reconstituted TAD-size chromatin fibers feature heterogeneous nucleosome clusters. SCIENTIFIC REPORTS 12 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-022-19471-3 | Abstract: | Large topologically associated domains (TADs) contain irregularly spaced nucleosome clutches, and interactions between such clutches are thought to aid the compaction of these domains. Here, we reconstituted TAD-sized chromatin fibers containing hundreds of nucleosomes on native source human and lambda-phage DNA and compared their mechanical properties at the single-molecule level with shorter ‘601’ arrays with various nucleosome repeat lengths. Fluorescent imaging showed increased compaction upon saturation of the DNA with histones and increasing magnesium concentration. Nucleosome clusters and their structural fluctuations were visualized in confined nanochannels. Force spectroscopy revealed not only similar mechanical properties of the TAD-sized fibers as shorter fibers but also large rupture events, consistent with breaking the interactions between distant clutches of nucleosomes. Though the arrays of native human DNA, lambda-phage and ‘601’ DNA featured minor differences in reconstitution yield and nucleosome stability, the fibers’ global structural and mechanical properties were similar, including the interactions between nucleosome clutches. These single-molecule experiments quantify the mechanical forces that stabilize large TAD-sized chromatin domains consisting of disordered, dynamically interacting nucleosome clutches and their effect on the condensation of large chromatin domains. | Source Title: | SCIENTIFIC REPORTS | URI: | https://scholarbank.nus.edu.sg/handle/10635/239172 | ISSN: | 2045-2322 | DOI: | 10.1038/s41598-022-19471-3 |
Appears in Collections: | Staff Publications Elements |
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