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Title: Integration of transcriptome and metabolome reveals the genes and metabolites involved in bifidobacterium bifidum biofilm formation
Authors: Liu, Zongmin
Li, Lingzhi
Fang, Zhifeng
Lee, Yuankun 
Zhao, Jianxin
Zhang, Hao
Chen, Wei
Li, Haitao
Lu, Wenwei
Keywords: Amino acid metabolism
Bifidobacterium bifidum
Quorum sensing
Two-component system
Issue Date: 15-Jul-2021
Publisher: MDPI
Citation: Liu, Zongmin, Li, Lingzhi, Fang, Zhifeng, Lee, Yuankun, Zhao, Jianxin, Zhang, Hao, Chen, Wei, Li, Haitao, Lu, Wenwei (2021-07-15). Integration of transcriptome and metabolome reveals the genes and metabolites involved in bifidobacterium bifidum biofilm formation. International Journal of Molecular Sciences 22 (14) : 7596. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Bifidobacterium bifidum strains, an important component of probiotic foods, can form bio-films on abiotic surfaces, leading to increased self-resistance. However, little is known about the molecular mechanism of B. bifidum biofilm formation. A time series transcriptome sequencing and untargeted metabolomics analysis of both B. bifidum biofilm and planktonic cells was performed to identify key genes and metabolites involved in biofilm formation. Two hundred thirty-five nonre-dundant differentially expressed genes (DEGs) (including vanY, pstS, degP, groS, infC, groL, yajC, tadB and sigA) and 219 nonredundant differentially expressed metabolites (including L-threonine, L-cystine, L-tyrosine, ascorbic acid, niacinamide, butyric acid and sphinganine) were identified. Thirteen pathways were identified during the integration of both transcriptomics and metabolomics data, including ABC transporters; quorum sensing; two-component system; oxidative phosphory-lation; cysteine and methionine metabolism; glutathione metabolism; glycine, serine and threonine metabolism; and valine, leucine and isoleucine biosynthesis. The DEGs that relate to the integration pathways included asd, atpB, degP, folC, ilvE, metC, pheA, pstS, pyrE, serB, ulaE, yajC and zwf. The differentially accumulated metabolites included L-cystine, L-serine, L-threonine, L-tyrosine, methylmalonate, monodehydroascorbate, nicotinamide, orthophosphate, spermine and tocopherol. These results indicate that quorum sensing, two-component system and amino acid metabolism are essential during B. bifidum biofilm formation. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: International Journal of Molecular Sciences
ISSN: 1661-6596
DOI: 10.3390/ijms22147596
Rights: Attribution 4.0 International
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