Please use this identifier to cite or link to this item: https://doi.org/10.3389/fmars.2021.655714
Title: Sodium-Dependent Phosphate Transporter Protein 1 Is Involved in the Active Uptake of Inorganic Phosphate in Nephrocytes of the Kidney and the Translocation of Pi Into the Tubular Epithelial Cells in the Outer Mantle of the Giant Clam, Tridacna squamosa
Authors: Ip, Yuen K. 
Boo, Mel, V 
Poo, Jeslyn S. T. 
Wong, Wai P. 
Chew, Shit F.
Keywords: photosynthesis
shell formation
Symbiodiniaceae
symbiosis
zooxanthellae
Issue Date: 31-Mar-2021
Publisher: Frontiers Media S.A.
Citation: Ip, Yuen K., Boo, Mel, V, Poo, Jeslyn S. T., Wong, Wai P., Chew, Shit F. (2021-03-31). Sodium-Dependent Phosphate Transporter Protein 1 Is Involved in the Active Uptake of Inorganic Phosphate in Nephrocytes of the Kidney and the Translocation of Pi Into the Tubular Epithelial Cells in the Outer Mantle of the Giant Clam, Tridacna squamosa. Frontiers in Marine Science 8 : 655714. ScholarBank@NUS Repository. https://doi.org/10.3389/fmars.2021.655714
Rights: Attribution 4.0 International
Abstract: Giant clams display light-enhanced inorganic phosphate (Pi) absorption, but how the absorbed Pi is translocated to the symbiotic dinoflagellates living extracellularly in a tubular system is unknown. They can accumulate Pi in the kidney, but the transport mechanism remains enigmatic. This study aimed to elucidate the possible functions of sodium-dependent phosphate transporter protein 1-homolog (PiT1-like), which co-transport Na+ and H2PO4–, in these two processes. The complete cDNA coding sequence of PiT1-like, which comprised 1,665 bp and encoded 553 amino acids (59.3 kDa), was obtained from the fluted giant clam, Tridacna squamosa. In the kidney, PiT1-like was localized in the plasma membrane of nephrocytes, and could therefore absorb Pi from the hemolymph. As the gene and protein expression levels of PiT1-like were up-regulated in the kidney during illumination, PiT1-like could probably increase the removal of Pi from the hemolymph during light-enhanced Pi uptake. In the ctenidial epithelial cells, PiT1-like had a basolateral localization and its expression was also light-dependent. It might function in Pi sensing and the absorption of Pi from the hemolymph when Pi was limiting. In the outer mantle, PiT1-like was localized in the basolateral membrane of epithelial cells forming the tertiary tubules. It displayed light-enhanced expression levels, indicating that the host could increase the translocation of Pi from the hemolymph into the tubular epithelial cells and subsequently into the luminal fluid to support increased Pi metabolism in the photosynthesizing dinoflagellates. Taken together, the accumulation of Pi in the kidney of giant clams might be unrelated to limiting the availability of Pi to the symbionts to regulate their population. © Copyright © 2021 Ip, Boo, Poo, Wong and Chew.
Source Title: Frontiers in Marine Science
URI: https://scholarbank.nus.edu.sg/handle/10635/232816
ISSN: 2296-7745
DOI: 10.3389/fmars.2021.655714
Rights: Attribution 4.0 International
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