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https://doi.org/10.1016/j.scr.2021.102238
| Title: | Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines | Authors: | Sims, C. H. Cole Autio, Matias, I Buskin, Adriana Cheung, Christine Heer, Rakesh Foo, Roger S. Y. Wang, Xin |
Issue Date: | 1-Apr-2021 | Publisher: | Elsevier B.V. | Citation: | Sims, C. H. Cole, Autio, Matias, I, Buskin, Adriana, Cheung, Christine, Heer, Rakesh, Foo, Roger S. Y., Wang, Xin (2021-04-01). Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines. Stem Cell Research 52 : 102238. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scr.2021.102238 | Rights: | Attribution-NonCommercial-NoDerivatives 4.0 International | Abstract: | Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans. © 2021 The Authors | Source Title: | Stem Cell Research | URI: | https://scholarbank.nus.edu.sg/handle/10635/232814 | ISSN: | 1873-5061 | DOI: | 10.1016/j.scr.2021.102238 | Rights: | Attribution-NonCommercial-NoDerivatives 4.0 International |
| Appears in Collections: | Elements Staff Publications |
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