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Title: Children with Plasmodium vivax infection previously observed in Namibia, were Duffy negative and carried a c.136G > A mutation
Authors: Haiyambo, Daniel Hosea
Aleksenko, Larysa
Mumbengegwi, Davies
Bock, Ronnie
Uusiku, Petrina
Malleret, Benoit 
Rénia, Laurent
Quaye, Isaac Kweku
Keywords: Duffy gene mutations
Plasmodium vivax
Issue Date: 21-Aug-2021
Publisher: BioMed Central Ltd
Citation: Haiyambo, Daniel Hosea, Aleksenko, Larysa, Mumbengegwi, Davies, Bock, Ronnie, Uusiku, Petrina, Malleret, Benoit, Rénia, Laurent, Quaye, Isaac Kweku (2021-08-21). Children with Plasmodium vivax infection previously observed in Namibia, were Duffy negative and carried a c.136G > A mutation. BMC Infectious Diseases 21 (1) : 856. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Background: In a previous study, using a molecular approach, we reported the presence of P. vivax in Namibia. Here, we have extended our investigation to the Duffy antigen genetic profile of individuals of the same cohort with and without Plasmodium infections. Methods: Participants with P. vivax (n = 3), P. falciparum (n = 23) mono-infections and co-infections of P. vivax/P. falciparum (n = 4), and P. falciparum/P. ovale (n = 3) were selected from seven regions. Participants with similar age but without any Plasmodium infections (n = 47) were also selected from all the regions. Duffy allelic profile was examined using standard PCR followed by sequencing of amplified products. Sequenced samples were also examined for the presence or absence of G125A mutation in codon 42, exon 2. Results: All individuals tested carried the ? 67 T > C mutation. However, while all P. vivax infected participants carried the c.G125A mutation, 7/28 P. falciparum infected participants and 9/41 of uninfected participants did not have the c.G125A mutation. The exon 2 region surrounding codon 42, had a c.136G > A mutation that was present in all P. vivax infections. The odds ratio for lack of this mutation with P. vivax infections was (OR 0.015, 95% CI 0.001–0.176; p = 0.001). Conclusion: We conclude that P. vivax infections previously reported in Namibia, occurred in Duffy negative participants, carrying the G125A mutation in codon 42. The role of the additional mutation c.136 G > A in exon 2 in P. vivax infections, will require further investigations. © 2021, The Author(s).
Source Title: BMC Infectious Diseases
ISSN: 1471-2334
DOI: 10.1186/s12879-021-06573-y
Rights: Attribution 4.0 International
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