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Title: Toxoplasma gondii SAG1 targeting host cell S100A6 for parasite invasion and host immunity
Authors: Zhou, Li-Juan
Peng, Jiao
Chen, Min
Yao, Li-Jie
Zou, Wei-Hao
He, Cynthia Y. 
Peng, Hong-Juan
Keywords: Immune response
Molecular biology
Issue Date: 1-Nov-2021
Publisher: Elsevier Inc.
Citation: Zhou, Li-Juan, Peng, Jiao, Chen, Min, Yao, Li-Jie, Zou, Wei-Hao, He, Cynthia Y., Peng, Hong-Juan (2021-11-01). Toxoplasma gondii SAG1 targeting host cell S100A6 for parasite invasion and host immunity. iScience 24 (12) : 103514. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: Toxoplasma gondii surface antigen 1 (TgSAG1) is a surface protein of tachyzoites, which plays a crucial role in toxoplasma gondii infection and host cell immune regulation. However, how TgSAG1 regulates these processes remains elucidated. We utilized the biotin ligase -TurboID fusion with TgSAG1 to identify the host proteins interacting with TgSAG1, and identified that S100A6 was co-localized with TgSAG1 when T. gondii attached to the host cell. S100A6, either knocking down or blocking its functional epitopes resulted in inhibited parasites invasion. Meanwhile, S100A6 overexpression in host cells promoted T. gondii infection. We further verified that TgSAG1 could inhibit the interaction of host cell vimentin with S100A6 for cytoskeleton organization during T. gondii invasion. As an immunogen, TgSAG1 could promote the secretion of tumor necrosis factor alpha (TNF-?) through S100A6-Vimentin/PKC?-NF-?B signaling pathway. In summary, our findings revealed a mechanism for how TgSAG1 functioned in parasitic invasion and host immune regulation. © 2021
Source Title: iScience
ISSN: 2589-0042
DOI: 10.1016/j.isci.2021.103514
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
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