Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms22189808
Title: Industrially compatible transfusable iPSC-derived RBCs: Progress, challenges and prospective solutions
Authors: Lim, Zhong Ri
Vassilev, Svetlan
Leong, Yew Wai
Hang, Jing Wen
Rénia, Laurent
Malleret, Benoit 
Oh, Steve Kah-Weng
Keywords: Bioprocess intensification
Enucleation
Erythropoiesis
Erythropoietic transcription factors
Genetic amenability
GMP-compliant
Hematopoietic stem cells
Human induced pluripotent stem cells
Reprogramming
Terminal maturation
Issue Date: 10-Sep-2021
Publisher: MDPI
Citation: Lim, Zhong Ri, Vassilev, Svetlan, Leong, Yew Wai, Hang, Jing Wen, Rénia, Laurent, Malleret, Benoit, Oh, Steve Kah-Weng (2021-09-10). Industrially compatible transfusable iPSC-derived RBCs: Progress, challenges and prospective solutions. International Journal of Molecular Sciences 22 (18) : 9808. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms22189808
Rights: Attribution 4.0 International
Abstract: Amidst the global shortfalls in blood supply, storage limitations of donor blood and the availability of potential blood substitutes for transfusion applications, society has pivoted towards in vitro generation of red blood cells (RBCs) as a means to solve these issues. Many conventional research studies over the past few decades have found success in differentiating hematopoietic stem and progenitor cells (HSPCs) from cord blood, adult bone marrow and peripheral blood sources. More recently, techniques that involve immortalization of erythroblast sources have also gained traction in tackling this problem. However, the RBCs generated from human induced pluripotent stem cells (hiPSCs) still remain as the most favorable solution due to many of its added advantages. In this review, we focus on the breakthroughs for high-density cultures of hiPSC-derived RBCs, and highlight the major challenges and prospective solutions throughout the whole process of erythropoiesis for hiPSC-derived RBCs. Furthermore, we elaborate on the recent advances and techniques used to achieve cost-effective, high-density cultures of GMP-compliant RBCs, and on their relevant novel applications after downstream processing and purification. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: International Journal of Molecular Sciences
URI: https://scholarbank.nus.edu.sg/handle/10635/232411
ISSN: 1661-6596
DOI: 10.3390/ijms22189808
Rights: Attribution 4.0 International
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