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Title: Optimisation of an aglycone?enhanced celery extract with germinated soy supplementation using response surface methodology
Authors: Lau, Hazel
Koh, Hui Min
Dayal, Hiranya 
Ren, Yi
Li, Sam Fong Yau 
Keywords: Aglycone
Germinated soy
Response surface methodology
Issue Date: 19-Oct-2021
Publisher: MDPI
Citation: Lau, Hazel, Koh, Hui Min, Dayal, Hiranya, Ren, Yi, Li, Sam Fong Yau (2021-10-19). Optimisation of an aglycone?enhanced celery extract with germinated soy supplementation using response surface methodology. Foods 10 (10) : 2505. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: In this study, the extraction conditions of bioactive aglycones from a celery extract supplemented with germinated soy were optimised by a response surface methodology. For subsequent enzymatic hydrolysis to enhance the apigenin content, increased production of its precursor apigetrin was firstly achieved through acidic extraction at optimal conditions, involving water at pH 1, at 75 °C for 2 h. Subsequently, a central composite design was conducted to analyse the pH (3–11) and temperature (25–35 °C) effects on the aglycone levels (apigenin, daidzein and genistein). The optimal extraction conditions were pH 7.02 and 29.99 °C, which resulted in a 40?fold increase in apigenin. The novel and cost?effective application of germinated soy ??glucosidase for the conversion of aglycones in non?soy foods is demonstrated. The enhanced bioactivities of aglycones may suggest potential applications for similar formulations as functional food ingredients. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Foods
ISSN: 2304-8158
DOI: 10.3390/foods10102505
Rights: Attribution 4.0 International
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