Please use this identifier to cite or link to this item:
Title: Understanding and exploiting the fatty acid desaturation system in Rhodotorula toruloides
Authors: Liu, Yanbin
Koh, Chong Mei John 
Yap, Sihui Amy 
Cai, Lin
Ji, Lianghui 
Keywords: Fatty acid desaturase
Palmitoleic acid
?-Linolenic acid
Issue Date: 19-Mar-2021
Publisher: BioMed Central Ltd
Citation: Liu, Yanbin, Koh, Chong Mei John, Yap, Sihui Amy, Cai, Lin, Ji, Lianghui (2021-03-19). Understanding and exploiting the fatty acid desaturation system in Rhodotorula toruloides. Biotechnology for Biofuels 14 (1) : 73. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Background: Rhodotorula toruloides is a robust producer of triacylglycerol owing to its fast growth rate and strong metabolic flux under conditions of high cell density fermentation. However, the molecular basis of fatty acid biosynthesis, desaturation and regulation remains elusive. Results: We present the molecular characterization of four fatty acid desaturase (FAD) genes in R. toruloides. Biosynthesis of oleic acid (OA) and palmitoleic acid (POA) was conferred by a single-copy ?9 Fad (Ole1) as targeted deletion of which abolished the biosynthesis of all unsaturated fatty acids. Conversion of OA to linoleic acid (LA) and ?-linolenic acid (ALA) was predominantly catalyzed by the bifunctional ?12/?15 Fad2. FAD4 was found to encode a trifunctional ?9/?12/?15 FAD, playing important roles in lipid and biomass production as well as stress resistance. Furthermore, an abundantly transcribed OLE1-related gene, OLE2 encoding a 149-aa protein, was shown to regulate Ole1 regioselectivity. Like other fungi, the transcription of FAD genes was controlled by nitrogen levels and fatty acids in the medium. A conserved DNA motif, (T/C)(G/A)TTGCAGA(T/C)CCCAG, was demonstrated to mediate the transcription of OLE1 by POA/OA. The applications of these FAD genes were illustrated by engineering high-level production of OA and ?-linolenic acid (GLA). Conclusion: Our work has gained novel insights on the transcriptional regulation of FAD genes, evolution of FAD enzymes and their roles in UFA biosynthesis, membrane stress resistance and, cell mass and total fatty acid production. Our findings should illuminate fatty acid metabolic engineering in R. toruloides and beyond. © 2021, The Author(s).
Source Title: Biotechnology for Biofuels
ISSN: 1754-6834
DOI: 10.1186/s13068-021-01924-y
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s13068-021-01924-y.pdf3.55 MBAdobe PDF




checked on Nov 29, 2022

Page view(s)

checked on Nov 17, 2022

Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons