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Title: Agrin-Matrix Metalloproteinase-12 axis confers a mechanically competent microenvironment in skin wound healing
Authors: Chakraborty, Sayan
Sampath, Divyaleka
Yu Lin, Melissa Ong
Bilton, Matthew
Huang, Cheng-Kuang 
Nai, Mui Hoon 
Njah, Kizito
Goy, Pierre-Alexis
Wang, Cheng-Chun
Guccione, Ernesto
Lim, Chwee-Teck 
Hong, Wanjin
Issue Date: 3-Nov-2021
Publisher: Nature Research
Citation: Chakraborty, Sayan, Sampath, Divyaleka, Yu Lin, Melissa Ong, Bilton, Matthew, Huang, Cheng-Kuang, Nai, Mui Hoon, Njah, Kizito, Goy, Pierre-Alexis, Wang, Cheng-Chun, Guccione, Ernesto, Lim, Chwee-Teck, Hong, Wanjin (2021-11-03). Agrin-Matrix Metalloproteinase-12 axis confers a mechanically competent microenvironment in skin wound healing. Nature Communications 12 (1) : 6349. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: An orchestrated wound healing program drives skin repair via collective epidermal cell proliferation and migration. However, the molecular determinants of the tissue microenvironment supporting wound healing remain poorly understood. Herein we discover that proteoglycan Agrin is enriched within the early wound-microenvironment and is indispensable for efficient healing. Agrin enhances the mechanoperception of keratinocytes by augmenting their stiffness, traction stress and fluidic velocity fields in retaliation to bulk substrate rigidity. Importantly, Agrin overhauls cytoskeletal architecture via enhancing actomyosin cables upon sensing geometric stress and force following an injury. Moreover, we identify Matrix Metalloproteinase-12 (MMP12) as a downstream effector of Agrin’s mechanoperception. We also reveal a promising potential of a recombinant Agrin fragment as a bio-additive material that assimilates optimal mechanobiological and pro-angiogenic parameters by engaging MMP12 in accelerated wound healing. Together, we propose that Agrin-MMP12 pathway integrates a broad range of mechanical stimuli to coordinate a competent skin wound healing niche. © 2021, The Author(s).
Source Title: Nature Communications
ISSN: 2041-1723
DOI: 10.1038/s41467-021-26717-7
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

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