Please use this identifier to cite or link to this item: https://doi.org/10.3389/fcimb.2021.691538
Title: Wide Application of Minimally Processed Saliva on Multiple RT-qPCR Kits for SARS-CoV-2 Detection in Indonesia
Authors: Mahendra, Caroline
Kaisar, Maria Mardalena Martini
Vasandani, Suraj Rajan
Surja, Sem Samuel
Tjoa, Enty
Chriestya, Febie
Junusmin, Kathleen Irena
Widowati, Tria Asri
Irwanto, Astrid 
Ali, Soegianto
Keywords: COVID-19
direct-PCR
large capacity
real-time PCR
RNA-extraction-free
saliva
SARS-CoV-2
stability
Issue Date: 18-Aug-2021
Publisher: Frontiers Media S.A.
Citation: Mahendra, Caroline, Kaisar, Maria Mardalena Martini, Vasandani, Suraj Rajan, Surja, Sem Samuel, Tjoa, Enty, Chriestya, Febie, Junusmin, Kathleen Irena, Widowati, Tria Asri, Irwanto, Astrid, Ali, Soegianto (2021-08-18). Wide Application of Minimally Processed Saliva on Multiple RT-qPCR Kits for SARS-CoV-2 Detection in Indonesia. Frontiers in Cellular and Infection Microbiology 11 : 691538. ScholarBank@NUS Repository. https://doi.org/10.3389/fcimb.2021.691538
Rights: Attribution 4.0 International
Abstract: Saliva as a sample matrix has been an attractive alternative for the detection of SARS-CoV-2. However, due to potential variability in collection and processing steps, evaluating a proposed workflow amongst the local population is recommended. Here, we aim to validate the collection and treatment of human saliva as a direct specimen for RT-qPCR-based detection of SARS-CoV-2 in Indonesia. We demonstrated that SARS-CoV-2 target genes were detected in saliva specimens and remained stable for five days either refrigerated or stored at room temperature. The method of processing saliva specimens described in this report bypasses the need for an RNA-extraction process, thereby reducing the cost, time, and manpower required for processing samples. The developed method was tested across nine commercial kits, including the benchmark, to demonstrate its wide applicability on multiple existing workflows. Our developed method achieved an 86% overall agreement rate compared to paired nasopharyngeal and oropharyngeal swab specimens (NPOP). With the assistance of a saliva sampling device, the collection was found to be more convenient for individuals and improved the overall agreement rate to 97%. © Copyright © 2021 Mahendra, Kaisar, Vasandani, Surja, Tjoa, Chriestya, Junusmin, Widowati, Irwanto and Ali.
Source Title: Frontiers in Cellular and Infection Microbiology
URI: https://scholarbank.nus.edu.sg/handle/10635/232037
ISSN: 2235-2988
DOI: 10.3389/fcimb.2021.691538
Rights: Attribution 4.0 International
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