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Title: AKTIP interacts with ESCRT i and is needed for the recruitment of ESCRT III subunits to the midbody
Authors: Merigliano, Chiara
Burla, Romina
La Torre, Mattia
Del Giudice, Simona
Teo, Hsiangling
Liew, Chong Wai
Chojnowski, Alexandre
Goh, Wah Ing
Olmos, Yolanda
Maccaroni, Klizia
Giubettini, Maria
Chiolo, Irene
Carlton, Jeremy G.
Raimondo, Domenico
Vernì, Fiammetta
Stewart, Colin L. 
Rhodes, Daniela
Wright, Graham D.
Burke, Brian E.
Saggio, Isabella
Issue Date: 27-Aug-2021
Publisher: Public Library of Science
Citation: Merigliano, Chiara, Burla, Romina, La Torre, Mattia, Del Giudice, Simona, Teo, Hsiangling, Liew, Chong Wai, Chojnowski, Alexandre, Goh, Wah Ing, Olmos, Yolanda, Maccaroni, Klizia, Giubettini, Maria, Chiolo, Irene, Carlton, Jeremy G., Raimondo, Domenico, Vernì, Fiammetta, Stewart, Colin L., Rhodes, Daniela, Wright, Graham D., Burke, Brian E., Saggio, Isabella (2021-08-27). AKTIP interacts with ESCRT i and is needed for the recruitment of ESCRT III subunits to the midbody. PLoS Genetics 17 (8) : e1009757. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: To complete mitosis, the bridge that links the two daughter cells needs to be cleaved. This step is carried out by the endosomal sorting complex required for transport (ESCRT) machinery. AKTIP, a protein discovered to be associated with telomeres and the nuclear membrane in interphase cells, shares sequence similarities with the ESCRT I component TSG101. Here we present evidence that during mitosis AKTIP is part of the ESCRT machinery at the midbody. AKTIP interacts with the ESCRT I subunit VPS28 and forms a circular supra-structure at the midbody, in close proximity with TSG101 and VPS28 and adjacent to the members of the ESCRT III module CHMP2A, CHMP4B and IST1. Mechanistically, the recruitment of AKTIP is dependent on MKLP1 and independent of CEP55. AKTIP and TSG101 are needed together for the recruitment of the ESCRT III subunit CHMP4B and in parallel for the recruitment of IST1. Alone, the reduction of AKTIP impinges on IST1 and causes multinucleation. Our data altogether reveal that AKTIP is a component of the ESCRT I module and functions in the recruitment of ESCRT III components required for abscission. © 2021 Merigliano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS Genetics
ISSN: 1553-7390
DOI: 10.1371/journal.pgen.1009757
Rights: Attribution 4.0 International
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