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Title: Multi-species single-cell transcriptomic analysis of ocular compartment regulons
Authors: Gautam, Pradeep
Hamashima, Kiyofumi
Chen, Ying 
Zeng, Yingying
Makovoz, Bar
Parikh, Bhav Harshad 
Lee, Hsin Yee
Lau, Katherine Anne
Su, Xinyi 
Wong, Raymond C. B.
Chan, Woon-Khiong 
Li, Hu
Blenkinsop, Timothy A.
Loh, Yuin-Han 
Issue Date: 28-Sep-2021
Publisher: Nature Research
Citation: Gautam, Pradeep, Hamashima, Kiyofumi, Chen, Ying, Zeng, Yingying, Makovoz, Bar, Parikh, Bhav Harshad, Lee, Hsin Yee, Lau, Katherine Anne, Su, Xinyi, Wong, Raymond C. B., Chan, Woon-Khiong, Li, Hu, Blenkinsop, Timothy A., Loh, Yuin-Han (2021-09-28). Multi-species single-cell transcriptomic analysis of ocular compartment regulons. Nature Communications 12 (1) : 5675. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The retina is a widely profiled tissue in multiple species by single-cell RNA sequencing studies. However, integrative research of the retina across species is lacking. Here, we construct the first single-cell atlas of the human and porcine ocular compartments and study inter-species differences in the retina. In addition to that, we identify putative adult stem cells present in the iris tissue. We also create a disease map of genes involved in eye disorders across compartments of the eye. Furthermore, we probe the regulons of different cell populations, which include transcription factors and receptor-ligand interactions and reveal unique directional signalling between ocular cell types. In addition, we study conservation of regulons across vertebrates and zebrafish to identify common core factors. Here, we show perturbation of KLF7 gene expression during retinal ganglion cells differentiation and conclude that it plays a significant role in the maturation of retinal ganglion cells. © 2021, The Author(s).
Source Title: Nature Communications
ISSN: 2041-1723
DOI: 10.1038/s41467-021-25968-8
Rights: Attribution 4.0 International
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