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https://doi.org/10.3892/ol.2017.5669
Title: | Single-cell genomic profiling of acute myeloid leukemia for clinical use: A pilot study | Authors: | Yan, Benedict Hu, Yongli Ban, Kenneth HK Tiang, Zenia Ng, Christopher Lee, Joanne Tan, Wilson Chiu, Lily Tan, Tin Wee Seah, Elaine Ng, Chin Hin Chng, Wee-Joo Foo, Roger |
Keywords: | Science & Technology Life Sciences & Biomedicine Oncology single cell genomics acute myeloid leukei transcriptomics gene expression RNA-SEQ FLOW-CYTOMETRY HETEROGENEITY TRANSCRIPTOME EXPRESSION |
Issue Date: | 1-Mar-2017 | Publisher: | SPANDIDOS PUBL LTD | Citation: | Yan, Benedict, Hu, Yongli, Ban, Kenneth HK, Tiang, Zenia, Ng, Christopher, Lee, Joanne, Tan, Wilson, Chiu, Lily, Tan, Tin Wee, Seah, Elaine, Ng, Chin Hin, Chng, Wee-Joo, Foo, Roger (2017-03-01). Single-cell genomic profiling of acute myeloid leukemia for clinical use: A pilot study. ONCOLOGY LETTERS 13 (3) : 1625-1630. ScholarBank@NUS Repository. https://doi.org/10.3892/ol.2017.5669 | Abstract: | Although bulk high-throughput genomic profiling studies have led to a significant increase in the understanding of cancer biology, there is increasing awareness that bulk profiling approaches do not completely elucidate tumor heterogeneity. Single-cell genomic profiling enables the distinction of tumor heterogeneity, and may improve clinical diagnosis through the identification and characterization of putative subclonal populations. In the present study, the challenges associated with a single-cell genomics profiling workflow for clinical diagnostics were investigated. Single-cell RNA-sequencing (RNA-seq) was performed on 20 cells from an acute myeloid leukemia bone marrow sample. Putative blasts were identified based on their gene expression profiles and principal component analysis was performed to identify outlier cells. Variant calling was performed on the single-cell RNA-seq data. The present pilot study demonstrates a proof of concept for clinical single-cell genomic profiling. The recognized limitations include significant stochastic RNA loss and the relatively low throughput of the current proposed platform. Although the results of the present study are promising, further technological advances and protocol. | Source Title: | ONCOLOGY LETTERS | URI: | https://scholarbank.nus.edu.sg/handle/10635/229090 | ISSN: | 17921074 17921082 |
DOI: | 10.3892/ol.2017.5669 |
Appears in Collections: | Staff Publications Elements |
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Single-cell genomic profiling of acute myeloid leukemia for clinical use A pilot study.pdf | Published version | 1.12 MB | Adobe PDF | CLOSED | None |
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