Please use this identifier to cite or link to this item: https://doi.org/10.3390/microorganisms9081724
Title: Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel
Authors: Zhang, Le 
Lim, Ee
Loh, Kai-Chee 
Dai, Yanjun
Tong, Yen 
Keywords: Science & Technology
Life Sciences & Biomedicine
Microbiology
two-stage fermentation
microbial lipids
biodiesel
oleaginous yeast
waste-to-resource
orange peel hydrolysate
YEAST YARROWIA-LIPOLYTICA
SINGLE-CELL OIL
OLEAGINOUS YEAST
LIGNOCELLULOSIC BIOMASS
CO-FERMENTATION
EXTRACTION
PRETREATMENT
GLUCOSE
OPTIMIZATION
CONVERSION
Issue Date: 1-Aug-2021
Publisher: MDPI
Citation: Zhang, Le, Lim, Ee, Loh, Kai-Chee, Dai, Yanjun, Tong, Yen (2021-08-01). Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel. MICROORGANISMS 9 (8). ScholarBank@NUS Repository. https://doi.org/10.3390/microorganisms9081724
Abstract: The high operating cost is currently a limitation to industrialize microbial lipids production by the yeast Lipomyces starkeyi. To explore economic fermentation technology, the two-stage fermentation of Lipomyces starkeyi using yeast extract peptone dextrose (YPD) medium, orange peel (OP) hydrolysate medium, and their mixed medium were investigated for seven days by monitoring OD600 values, pH values, cell growth status, C/N ratios, total carbon concentration, total nitrogen concentration, residual sugar concentration, lipid content, lipid titer, and fatty acids profiles of lipids. The results showed that two-stage fermentation with YPD and 50% YPD + 50% OP medium contributed to lipid accumulation, leading to larger internal lipid droplets in the yeast cells. However, the cells in pure OP hydrolysate grew abnormally, showing skinny and angular shapes. Compared to the one-stage fermentation, the two-stage fermentation enhanced lipid contents by 18.5%, 27.1%, and 21.4% in the flasks with YPD medium, OP medium, and 50%YPD + 50%OP medium, and enhanced the lipid titer by 77.8%, 13.6%, and 63.0%, respectively. The microbial lipids obtained from both one-stage and two-stage fermentation showed no significant difference in fatty acid compositions, which were mainly dominated by palmitic acid (33.36–38.43%) and oleic acid (46.6–48.12%). Hence, a mixture of commercial medium and lignocellulosic biomass hydrolysate could be a promising option to balance the operating cost and lipid production.
Source Title: MICROORGANISMS
URI: https://scholarbank.nus.edu.sg/handle/10635/229038
ISSN: 20762607
DOI: 10.3390/microorganisms9081724
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel. .pdfPublished version2.81 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

3
checked on Sep 23, 2022

Page view(s)

21
checked on Sep 22, 2022

Download(s)

3
checked on Sep 22, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.