Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41698-019-0102-5
Title: Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip
Authors: Khoo, Bee Luan
Shang, Menglin
Ng, Chin Hin
Lim, Chwee Teck 
Chng, Wee Joo 
Han, Jongyoon
Keywords: Science & Technology
Life Sciences & Biomedicine
Oncology
ACUTE MYELOID-LEUKEMIA
CIRCULATING TUMOR-CELLS
LABEL-FREE ISOLATION
BONE-MARROW
ULTRA-FAST
MICROFLUIDICS
DEFORMABILITY
Issue Date: 2-Dec-2019
Publisher: NATURE PUBLISHING GROUP
Citation: Khoo, Bee Luan, Shang, Menglin, Ng, Chin Hin, Lim, Chwee Teck, Chng, Wee Joo, Han, Jongyoon (2019-12-02). Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip. NPJ PRECISION ONCOLOGY 3 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s41698-019-0102-5
Abstract: Long-term management for leukemia is challenging due to the painful and invasive procedure of bone marrow (BM) biopsy. At present, non-invasive liquid (blood) biopsy is not utilized for leukemia, due to lower counts of leukemia blast cells in the blood. Here, we described a robust system for the simultaneous detection and enrichment of rare blast cells. Enrichment of blast cells was achieved from blood with a one-step microfluidic blast cell biochip (BCB) sorting system, without specific targeting of proteins by antibodies. Non-target cells encountered a differential net force as compared to stiffer blast cells and were removed. The efficiency of the BCB promotes high detection sensitivity (1 in 106 cells) even from patients with minimal residual disease. The procedure was validated using actual blast cells from patients with various types of leukemia. Outcomes were compared to current evaluation standards, such as flow cytometry, using BM aspirates. Blast cell detection efficiency was higher in 55.6% of the patients using the BCB as compared to flow cytometry, despite the lower concentrations of blast cells in liquid biopsy. These studies promote early-stage detection and routine monitoring for minimal residual disease in patients.
Source Title: NPJ PRECISION ONCOLOGY
URI: https://scholarbank.nus.edu.sg/handle/10635/229008
ISSN: 2397768X
DOI: 10.1038/s41698-019-0102-5
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