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https://doi.org/10.1016/j.cell.2016.11.020
Title: | Modular Assembly of the Bacterial Large Ribosomal Subunit | Authors: | Davis, Joseph H Tan, Yong Zi Carragher, Bridget Potter, Clinton S Lyumkis, Dmitry Williamson, James R |
Keywords: | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Cell Biology QUANTITATIVE MASS-SPECTROMETRY PARTICLE CRYO-EM ELECTRON-MICROSCOPY DATABASE SEARCH RESOLUTION MS/MS LANDSCAPE FREALIGN LIBRARY GENES |
Issue Date: | 1-Dec-2016 | Publisher: | CELL PRESS | Citation: | Davis, Joseph H, Tan, Yong Zi, Carragher, Bridget, Potter, Clinton S, Lyumkis, Dmitry, Williamson, James R (2016-12-01). Modular Assembly of the Bacterial Large Ribosomal Subunit. CELL 167 (6) : 1610-+. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cell.2016.11.020 | Abstract: | The ribosome is a complex macromolecular machine and serves as an ideal system for understanding biological macromolecular assembly. Direct observation of ribosome assembly in vivo is difficult, as few intermediates have been isolated and thoroughly characterized. Herein, we deploy a genetic system to starve cells of an essential ribosomal protein, which results in the accumulation of assembly intermediates that are competent for maturation. Quantitative mass spectrometry and single-particle cryo-electron microscopy reveal 13 distinct intermediates, which were each resolved to ∼4–5 Å resolution and could be placed in an assembly pathway. We find that ribosome biogenesis is a parallel process, that blocks of structured rRNA and proteins assemble cooperatively, and that the entire process is dynamic and can be “re-routed” through different pathways as needed. This work reveals the complex landscape of ribosome assembly in vivo and provides the requisite tools to characterize additional assembly pathways for ribosomes and other macromolecular machines. | Source Title: | CELL | URI: | https://scholarbank.nus.edu.sg/handle/10635/227219 | ISSN: | 00928674 10974172 |
DOI: | 10.1016/j.cell.2016.11.020 |
Appears in Collections: | Staff Publications Elements |
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2016-12-01_Davis_Cell.pdf | Published version | 9.89 MB | Adobe PDF | CLOSED | None |
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