Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.cell.2016.11.020
Title: Modular Assembly of the Bacterial Large Ribosomal Subunit
Authors: Davis, Joseph H
Tan, Yong Zi 
Carragher, Bridget
Potter, Clinton S
Lyumkis, Dmitry
Williamson, James R
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
QUANTITATIVE MASS-SPECTROMETRY
PARTICLE CRYO-EM
ELECTRON-MICROSCOPY
DATABASE SEARCH
RESOLUTION
MS/MS
LANDSCAPE
FREALIGN
LIBRARY
GENES
Issue Date: 1-Dec-2016
Publisher: CELL PRESS
Citation: Davis, Joseph H, Tan, Yong Zi, Carragher, Bridget, Potter, Clinton S, Lyumkis, Dmitry, Williamson, James R (2016-12-01). Modular Assembly of the Bacterial Large Ribosomal Subunit. CELL 167 (6) : 1610-+. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cell.2016.11.020
Abstract: The ribosome is a complex macromolecular machine and serves as an ideal system for understanding biological macromolecular assembly. Direct observation of ribosome assembly in vivo is difficult, as few intermediates have been isolated and thoroughly characterized. Herein, we deploy a genetic system to starve cells of an essential ribosomal protein, which results in the accumulation of assembly intermediates that are competent for maturation. Quantitative mass spectrometry and single-particle cryo-electron microscopy reveal 13 distinct intermediates, which were each resolved to ∼4–5 Å resolution and could be placed in an assembly pathway. We find that ribosome biogenesis is a parallel process, that blocks of structured rRNA and proteins assemble cooperatively, and that the entire process is dynamic and can be “re-routed” through different pathways as needed. This work reveals the complex landscape of ribosome assembly in vivo and provides the requisite tools to characterize additional assembly pathways for ribosomes and other macromolecular machines.
Source Title: CELL
URI: https://scholarbank.nus.edu.sg/handle/10635/227219
ISSN: 00928674
10974172
DOI: 10.1016/j.cell.2016.11.020
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