Please use this identifier to cite or link to this item: https://doi.org/10.1038/NMETH.4347
Title: Addressing preferred specimen orientation in single-particle cryo-EM through tilting
Authors: Tan, Yong Zi 
Baldwin, Philip R
Davis, Joseph H
Williamson, James R
Potter, Clinton S
Carragher, Bridget
Lyumkis, Dmitry
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
AB-INITIO RECONSTRUCTION
ELECTRON-MICROSCOPY
CRYOELECTRON MICROSCOPY
MISSING-CONE
ALGORITHMS
CRYOMICROSCOPY
ENVIRONMENT
TOMOGRAPHY
FREALIGN
SUBUNIT
Issue Date: 1-Aug-2017
Publisher: NATURE PUBLISHING GROUP
Citation: Tan, Yong Zi, Baldwin, Philip R, Davis, Joseph H, Williamson, James R, Potter, Clinton S, Carragher, Bridget, Lyumkis, Dmitry (2017-08-01). Addressing preferred specimen orientation in single-particle cryo-EM through tilting. NATURE METHODS 14 (8) : 793-+. ScholarBank@NUS Repository. https://doi.org/10.1038/NMETH.4347
Abstract: We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-Atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adopt moderately preferred orientations.
Source Title: NATURE METHODS
URI: https://scholarbank.nus.edu.sg/handle/10635/227218
ISSN: 15487091
15487105
DOI: 10.1038/NMETH.4347
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