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https://doi.org/10.1016/j.jsb.2018.01.002
| Title: | Spotiton: New features and applications | Authors: | Dandey, Venkata P Wei, Hui Zhang, Zhening Tan, Yong Zi Acharya, Priyamvada Eng, Edward T Rice, William J Kahn, Peter A Potter, Clinton S Carragher, Bridget |
Keywords: | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Biophysics Cell Biology cryoEM vitrification piezo dispensing nanowire grids PARTICLE CRYO-EM CRYOELECTRON MICROSCOPY ELECTRON-MICROGRAPHS SAMPLES SYSTEM TEM |
Issue Date: | 1-May-2018 | Publisher: | ACADEMIC PRESS INC ELSEVIER SCIENCE | Citation: | Dandey, Venkata P, Wei, Hui, Zhang, Zhening, Tan, Yong Zi, Acharya, Priyamvada, Eng, Edward T, Rice, William J, Kahn, Peter A, Potter, Clinton S, Carragher, Bridget (2018-05-01). Spotiton: New features and applications. JOURNAL OF STRUCTURAL BIOLOGY 202 (2) : 161-169. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jsb.2018.01.002 | Abstract: | We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ∼3 Å resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples. | Source Title: | JOURNAL OF STRUCTURAL BIOLOGY | URI: | https://scholarbank.nus.edu.sg/handle/10635/227216 | ISSN: | 10478477 10958657 |
DOI: | 10.1016/j.jsb.2018.01.002 |
| Appears in Collections: | Staff Publications Elements |
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| 2018-01-04_Dandey_Journalofstructuralbiology.pdf | Published version | 4.86 MB | Adobe PDF | CLOSED | None |
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