Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-019-10779-9
Title: The structure of a 15-stranded actin-like filament from Clostridium botulinum
Authors: Koh, Fujiet
Narita, Akihiro
Lee, Lin Jie
Tanaka, Kotaro
Tan, Yong Zi 
Dandey, Venkata P
Popp, David
Robinson, Robert C
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
CRYO-EM STRUCTURE
PLASMID
SEGREGATION
DYNAMICS
COMPLEX
DEFOCUS
SYSTEM
PHENIX
MODEL
Issue Date: 28-Jun-2019
Publisher: NATURE PUBLISHING GROUP
Citation: Koh, Fujiet, Narita, Akihiro, Lee, Lin Jie, Tanaka, Kotaro, Tan, Yong Zi, Dandey, Venkata P, Popp, David, Robinson, Robert C (2019-06-28). The structure of a 15-stranded actin-like filament from Clostridium botulinum. NATURE COMMUNICATIONS 10 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-019-10779-9
Abstract: Microfilaments (actin) and microtubules represent the extremes in eukaryotic cytoskeleton cross-sectional dimensions, raising the question of whether filament architectures are limited by protein fold. Here, we report the cryoelectron microscopy structure of a complex filament formed from 15 protofilaments of an actin-like protein. This actin-like ParM is encoded on the large pCBH Clostridium botulinum plasmid. In cross-section, the ~26 nm diameter filament comprises a central helical protofilament surrounded by intermediate and outer layers of six and eight twisted protofilaments, respectively. Alternating polarity of the layers allows for similar lateral contacts between each layer. This filament design is stiffer than the actin filament, and has likely been selected for during evolution to move large cargos. The comparable sizes of microtubule and pCBH ParM filaments indicate that larger filament architectures are not limited by the protomer fold. Instead, function appears to have been the evolutionary driving force to produce broad, complex filaments.
Source Title: NATURE COMMUNICATIONS
URI: https://scholarbank.nus.edu.sg/handle/10635/227208
ISSN: 20411723
DOI: 10.1038/s41467-019-10779-9
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