Please use this identifier to cite or link to this item: https://doi.org/10.18632/aging.101181
Title: Changes in macroautophagy, chaperone-mediated autophagy, and mitochondrial metabolism in murine skeletal and cardiac muscle during aging
Authors: Zhou, Jin 
Chong, Shu Yun 
Lim, Andrea 
Singh, Brijesh K 
Sinha, Rohit A 
Salmon, Adam B
Yen, Paul M 
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell Biology
Geriatrics & Gerontology
aging
muscle
heart
autophagy
chaperone-mediated autophagy (CMA)
fatty acid oxidation
ceramide
INSULIN-RESISTANCE
DISEASE
HUMANS
DYSFUNCTION
STRESS
OBESE
STIMULATION
INHIBITION
LIPIDATION
SIGNATURE
Issue Date: 1-Feb-2017
Publisher: IMPACT JOURNALS LLC
Citation: Zhou, Jin, Chong, Shu Yun, Lim, Andrea, Singh, Brijesh K, Sinha, Rohit A, Salmon, Adam B, Yen, Paul M (2017-02-01). Changes in macroautophagy, chaperone-mediated autophagy, and mitochondrial metabolism in murine skeletal and cardiac muscle during aging. AGING-US 9 (2) : 583-599. ScholarBank@NUS Repository. https://doi.org/10.18632/aging.101181
Abstract: Aging causes a general decline in cellular metabolic activity, and function in different tissues and whole body homeostasis. However, the understanding about the metabolomic and autophagy changes in skeletal muscle and heart during aging is still limited. We thus examined markers for macroautophagy, chaperone-mediated autophagy (CMA), mitochondrial quality control, as well as cellular metabolites in skeletal and cardiac muscle from young (5 months old) and aged (27 months old) mice. We found decreased autophagic degradation of p62 and increased ubiquitinated proteins in both tissues from aged mice, suggesting a decline in macroautophagy during aging. In skeletal muscle from aged mice, there also was a decline in LC3B-I conjugation to phosphatidylethanolamine (PE) possibly due to decreased protein levels of ATG3 and ATG12-ATG5. The CMA markers, LAMP-2A and Hsc70, and mitochondrial turnover markers, Drp1, PINK1 and PGC1a also were decreased. Metabolomics analysis showed impaired β-oxidation in heart of aged mice, whereas increased branched-chain amino acids (BCAAs) and ceramide levels were found in skeletal muscle of aged mice that in turn, may contribute to insulin resistance in muscle. Taken together, our studies showed similar declines in macroautophagy but distinct effects on CMA, mitochondrial turnover, and metabolic dysfunction in muscle vs. heart during aging.
Source Title: AGING-US
URI: https://scholarbank.nus.edu.sg/handle/10635/226758
ISSN: 19454589
DOI: 10.18632/aging.101181
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