Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.xpro.2020.100064
Title: Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging
Authors: Tripathi, M 
Yen, PM 
Singh, BK 
Keywords: Animals
Cell Culture Techniques
Cell Line
Cell Separation
Cellular Senescence
Hepatocytes
Liver
Mice
Issue Date: 18-Sep-2020
Publisher: Elsevier BV
Citation: Tripathi, M, Yen, PM, Singh, BK (2020-09-18). Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging. STAR Protocols 1 (2) : 100064-. ScholarBank@NUS Repository. https://doi.org/10.1016/j.xpro.2020.100064
Abstract: Previously developed senescent primary fibroblast models have limited relevance to study age-related changes in metabolically active tissues such as the liver. Here, we describe a protocol to generate senescent cells from the mouse hepatic cell line, AML12. These senescent cells exhibit molecular and metabolic signatures that are similar to those observed in livers from aged mice. These senescent AML12 cells should be a useful in vitro model to study the metabolic effects of aging in the liver. For complete details on the use and execution of this protocol, please refer to Singh et al. (2020).
Source Title: STAR Protocols
URI: https://scholarbank.nus.edu.sg/handle/10635/226716
ISSN: 26661667
DOI: 10.1016/j.xpro.2020.100064
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