Please use this identifier to cite or link to this item: https://doi.org/10.1002/adfm.202004068
Title: Gram-Typing Using Conjugated Oligoelectrolytes
Authors: ZHOU CHENG 
Ho, James CS
Chia, Geraldine WN
Moreland, Alex S
Ruan, Lin
Liedberg, Bo
Kjelleberg, Staffan
Hinks, Jamie
Bazan Guillermo Carlos 
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
biofilm imaging
conjugated oligoelectrolytes
Gram staining
lipopolysaccharides
naked eye detection
CELL-WALLS
STAIN
BIOFILMS
AGGREGATION
POLYMERS
PROBE
Issue Date: 19-Aug-2020
Publisher: WILEY-V C H VERLAG GMBH
Citation: ZHOU CHENG, Ho, James CS, Chia, Geraldine WN, Moreland, Alex S, Ruan, Lin, Liedberg, Bo, Kjelleberg, Staffan, Hinks, Jamie, Bazan Guillermo Carlos (2020-08-19). Gram-Typing Using Conjugated Oligoelectrolytes. ADVANCED FUNCTIONAL MATERIALS 30 (42). ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.202004068
Abstract: It is shown that Gram-type differentiation, an essential tool in microbiology, can now be easily accomplished by using the chain-elongated conjugated oligoelectrolyte COE-S6. Specifically, COE-S6 can be used as a fluorescent membrane probe that distinguishes between Gram-negative and Gram-positive bacteria in a single step. Confocal microscopy of labeled microorganisms and differential scanning calorimetry with phospholipid- and lipopolysaccharide-containing model membranes suggest that lipopolysaccharides impede COE-S6 membrane intercalation in Gram-negative bacteria. Both Gram-types within a mixture are discretely labeled by counterstaining COE-S6 with the nonspecific and lipophilic membrane probe FM 4–64. This single-step procedure allows for the in situ visualization of individual Gram-types in complex polymicrobial biofilms. Given that COE-S6 fluorescence intensity increases considerably post intercalation, it can be used for the detection of Gram-positive bacteria by the naked eye. COE-S6 does not inhibit bacterial growth and is simple to use, making it a promising membrane-specific fluorescent probe.
Source Title: ADVANCED FUNCTIONAL MATERIALS
URI: https://scholarbank.nus.edu.sg/handle/10635/217436
ISSN: 1616-301X
1616-3028
DOI: 10.1002/adfm.202004068
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