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https://doi.org/10.1021/acs.jpclett.0c02251
Title: | Role of Internal DNA Motion on the Mobility of a Nucleoid-Associated Protein | Authors: | Yadav, Indresh Basak, Rajib Yan, Peiyan van Kan, Jeroen A Arluison, Veronique van der Maarel, Johan RC |
Keywords: | Science & Technology Physical Sciences Technology Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Atomic, Molecular & Chemical Chemistry Science & Technology - Other Topics Materials Science Physics SINGLE DNA FACILITATED DIFFUSION RNA-POLYMERASE HFQ COMPACTION ELONGATION MOLECULES DYNAMICS BINDING SEARCH |
Issue Date: | 1-Oct-2020 | Publisher: | AMER CHEMICAL SOC | Citation: | Yadav, Indresh, Basak, Rajib, Yan, Peiyan, van Kan, Jeroen A, Arluison, Veronique, van der Maarel, Johan RC (2020-10-01). Role of Internal DNA Motion on the Mobility of a Nucleoid-Associated Protein. JOURNAL OF PHYSICAL CHEMISTRY LETTERS 11 (19) : 8424-8429. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.jpclett.0c02251 | Abstract: | Protein transport on DNA is at the core of the machinery of life. Here we investigated the influence of DNA internal motion on the mobility of Hfq, which is involved in several aspects of nucleic acid metabolism and is one of the nucleoid-associated proteins that shape the bacterial chromosome. Fluorescence microscopy was used to follow Hfq on doublestranded DNA that was stretched by confinement to a channel with a diameter of 125 nm. The protein mobility shows a strong dependence on the internal motion of DNA in that slower motion results in faster protein diffusion. A model of released diffusion is proposed that is based on three-dimensional diffusion through the interior of the DNA coil interspersed by periods in which the protein is immobilized in a bound state. We surmise that the coupling between DNA internal motion and protein mobility has important implications for DNA metabolism and protein-binding-related regulation of gene expression. | Source Title: | JOURNAL OF PHYSICAL CHEMISTRY LETTERS | URI: | https://scholarbank.nus.edu.sg/handle/10635/215536 | ISSN: | 19487185 | DOI: | 10.1021/acs.jpclett.0c02251 |
Appears in Collections: | Staff Publications Elements |
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