Please use this identifier to cite or link to this item: https://doi.org/10.1021/acs.jpclett.0c02251
Title: Role of Internal DNA Motion on the Mobility of a Nucleoid-Associated Protein
Authors: Yadav, Indresh 
Basak, Rajib 
Yan, Peiyan
van Kan, Jeroen A 
Arluison, Veronique
van der Maarel, Johan RC 
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Atomic, Molecular & Chemical
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
SINGLE DNA
FACILITATED DIFFUSION
RNA-POLYMERASE
HFQ
COMPACTION
ELONGATION
MOLECULES
DYNAMICS
BINDING
SEARCH
Issue Date: 1-Oct-2020
Publisher: AMER CHEMICAL SOC
Citation: Yadav, Indresh, Basak, Rajib, Yan, Peiyan, van Kan, Jeroen A, Arluison, Veronique, van der Maarel, Johan RC (2020-10-01). Role of Internal DNA Motion on the Mobility of a Nucleoid-Associated Protein. JOURNAL OF PHYSICAL CHEMISTRY LETTERS 11 (19) : 8424-8429. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.jpclett.0c02251
Abstract: Protein transport on DNA is at the core of the machinery of life. Here we investigated the influence of DNA internal motion on the mobility of Hfq, which is involved in several aspects of nucleic acid metabolism and is one of the nucleoid-associated proteins that shape the bacterial chromosome. Fluorescence microscopy was used to follow Hfq on doublestranded DNA that was stretched by confinement to a channel with a diameter of 125 nm. The protein mobility shows a strong dependence on the internal motion of DNA in that slower motion results in faster protein diffusion. A model of released diffusion is proposed that is based on three-dimensional diffusion through the interior of the DNA coil interspersed by periods in which the protein is immobilized in a bound state. We surmise that the coupling between DNA internal motion and protein mobility has important implications for DNA metabolism and protein-binding-related regulation of gene expression.
Source Title: JOURNAL OF PHYSICAL CHEMISTRY LETTERS
URI: https://scholarbank.nus.edu.sg/handle/10635/215536
ISSN: 19487185
DOI: 10.1021/acs.jpclett.0c02251
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
jpclett.0c02251.pdfPublished version3.74 MBAdobe PDF

CLOSED

None

SCOPUSTM   
Citations

4
checked on Sep 23, 2022

Page view(s)

64
checked on Sep 22, 2022

Download(s)

1
checked on Sep 22, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.