Please use this identifier to cite or link to this item: https://doi.org/10.1021/acs.biomac.0c00747
Title: Interactions between DNA and the Hfq Amyloid-like Region Trigger a Viscoelastic Response
Authors: El Hamoui, Omar
Yadav, Indresh 
Radiom, Milad
Wien, Frank
Berret, Jean-Francois
van der Maarel, Johan RC 
Arluison, Veronique
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemistry & Molecular Biology
Chemistry, Organic
Polymer Science
Chemistry
ESCHERICHIA-COLI-CELLS
TRANSCRIPTION FACTOR
PROTEINS
DYNAMICS
COMPACTION
CONDENSATION
TRANSLATION
CHROMOSOME
TRANSITION
MECHANISM
Issue Date: 1-Sep-2020
Publisher: AMER CHEMICAL SOC
Citation: El Hamoui, Omar, Yadav, Indresh, Radiom, Milad, Wien, Frank, Berret, Jean-Francois, van der Maarel, Johan RC, Arluison, Veronique (2020-09-01). Interactions between DNA and the Hfq Amyloid-like Region Trigger a Viscoelastic Response. BIOMACROMOLECULES 21 (9) : 3668-3677. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.biomac.0c00747
Abstract: Molecular transport of biomolecules plays a pivotal role in the machinery of life. Yet, this role is poorly understood due the lack of quantitative information. Here, the role and properties of the C-terminal region of Escherichia coli Hfq is reported, involved in controlling the flow of a DNA solution. A combination of experimental methodologies has been used to probe the interaction of Hfq with DNA and to measure the rheological properties of the complex. A physical gel with a temperature reversible elasticity modulus is formed due to the formation of noncovalent cross-links. The mechanical response of the complexes shows that they are inhomogeneous soft solids. Our experiments indicate that the Hfq C-terminal region could contribute to the genome's mechanical response. The reported viscoelasticity of the DNA-protein complex might have implications for cellular processes involving molecular transport of DNA or segments thereof.
Source Title: BIOMACROMOLECULES
URI: https://scholarbank.nus.edu.sg/handle/10635/215518
ISSN: 15257797
15264602
DOI: 10.1021/acs.biomac.0c00747
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