Please use this identifier to cite or link to this item: https://doi.org/10.1155/2019/4616034
Title: Stimulation of Na +/K +-ATPase with an Antibody against Its 4th Extracellular Region Attenuates Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy via an AMPK/SIRT3/PPAR γ Signaling Pathway
Authors: Xiong, S. 
Sun, H.-J. 
Cao, L. 
Zhu, M.
Liu, T. 
Wu, Z. 
Bian, J.-S. 
Issue Date: 2019
Publisher: Hindawi Limited
Citation: Xiong, S., Sun, H.-J., Cao, L., Zhu, M., Liu, T., Wu, Z., Bian, J.-S. (2019). Stimulation of Na +/K +-ATPase with an Antibody against Its 4th Extracellular Region Attenuates Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy via an AMPK/SIRT3/PPAR γ Signaling Pathway. Oxidative Medicine and Cellular Longevity 2019 : 4616034. ScholarBank@NUS Repository. https://doi.org/10.1155/2019/4616034
Rights: Attribution 4.0 International
Abstract: Activation of the renin-angiotensin system (RAS) contributes to the pathogenesis of cardiovascular diseases. Sodium potassium ATPase (NKA) expression and activity are often regulated by angiotensin II (Ang II). This study is aimed at investigating whether DR-Ab, an antibody against 4th extracellular region of NKA, can protect Ang II-induced cardiomyocyte hypertrophy. Our results showed that Ang II treatment significantly reduced NKA activity and membrane expression. Pretreatment with DR-Ab preserved cell size in Ang II-induced cardiomyopathy by stabilizing the plasma membrane expression of NKA and restoring its activity. DR-Ab reduced intracellular ROS generation through inhibition of NADPH oxidase activity and protection of mitochondrial functions in Ang II-treated H9c2 cardiomyocytes. Pharmacological manipulation and Western blotting analysis demonstrated the cardioprotective effects were mediated by the activation of the AMPK/Sirt-3/PPARγ signaling pathway. Taken together, our results suggest that dysfunction of NKA is an important mechanism for Ang II-induced cardiomyopathy and DR-Ab may be a novel and promising therapeutic approach to treat cardiomyocyte hypertrophy. © 2019 Siping Xiong et al.
Source Title: Oxidative Medicine and Cellular Longevity
URI: https://scholarbank.nus.edu.sg/handle/10635/213720
ISSN: 1942-0900
DOI: 10.1155/2019/4616034
Rights: Attribution 4.0 International
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