Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.isci.2019.05.019
Title: Evolution of Subgenomic RNA Shapes Dengue Virus Adaptation and Epidemiological Fitness
Authors: Finol, E. 
Ooi, E.E. 
Keywords: Bioinformatics
Evolutionary Mechanisms
Phylogenetics
Virology
Issue Date: 2019
Publisher: Elsevier Inc.
Citation: Finol, E., Ooi, E.E. (2019). Evolution of Subgenomic RNA Shapes Dengue Virus Adaptation and Epidemiological Fitness. iScience 16 : 94-105. ScholarBank@NUS Repository. https://doi.org/10.1016/j.isci.2019.05.019
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: Virology; Evolutionary Mechanisms; Bioinformatics; Phylogenetics © 2019 The Author(s)Changes in dengue virus (DENV)genome affect viral fitness both clinically and epidemiologically. Even in the 3? untranslated region (3? UTR), mutations could affect subgenomic flaviviral RNA (sfRNA)production and its affinity for host proteins, which are necessary for successful viral replication. Indeed, we recently showed that mutations in DENV2 3? UTR of epidemic strains increased sfRNA ability to bind host proteins and reduce interferon expression. However, whether 3? UTR differences shape the overall DENV evolution remains incompletely understood. Herein, we combined RNA phylogeny with phylogenetics to gain insights on sfRNA evolution. We found that sfRNA structures are under purifying selection and highly conserved despite sequence divergence. Only the second flaviviral nuclease-resistant RNA (fNR2)structure of DENV2 sfRNA has undergone strong positive selection. Epidemiological reports suggest that substitutions in fNR2 may drive DENV2 epidemiological fitness, possibly through sfRNA-protein interactions. Collectively, our findings indicate that 3? UTRs are important determinants of DENV fitness in human-mosquito cycles. © 2019 The Author(s)
Source Title: iScience
URI: https://scholarbank.nus.edu.sg/handle/10635/212458
ISSN: 2589-0042
DOI: 10.1016/j.isci.2019.05.019
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
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