Please use this identifier to cite or link to this item:
https://doi.org/10.3390/molecules24040714
Title: | Identification of Phytoconstituents in Leea indica (Burm. F.) Merr. Leaves by high performance liquid chromatography micro time-of-flight mass spectrometry | Authors: | Singh, D. Siew, Y.-Y. Chong, T.-I. Yew, H.-C. Ho, S.S.-W. Lim, C.S.E.-S. Tan, W.-X. Neo, S.-Y. Koh, H.-L. |
Keywords: | Dihydrochalcones HPLC-ESI-microTOF-Q-MS/MS Leea indica Phenolics |
Issue Date: | 2019 | Publisher: | MDPI AG | Citation: | Singh, D., Siew, Y.-Y., Chong, T.-I., Yew, H.-C., Ho, S.S.-W., Lim, C.S.E.-S., Tan, W.-X., Neo, S.-Y., Koh, H.-L. (2019). Identification of Phytoconstituents in Leea indica (Burm. F.) Merr. Leaves by high performance liquid chromatography micro time-of-flight mass spectrometry. Molecules 24 (4) : 714. ScholarBank@NUS Repository. https://doi.org/10.3390/molecules24040714 | Rights: | Attribution 4.0 International | Abstract: | Leea indica (Vitaceae) is a Southeast Asian medicinal plant. In this study, an ethyl acetate fraction of L. indica leaves was studied for its phytoconstituents using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-microTOF-Q-MS/MS) analysis. A total of 31 compounds of different classes, including benzoic acid derivatives, phenolics, flavonoids, catechins, dihydrochalcones, coumarins, megastigmanes, and oxylipins were identified using LC-MS/MS. Among them, six compounds including gallic acid, methyl gallate, (?)-epigallocatechin-3-O-gallate, myricetin-3-O-rhamnoside, quercetin-3-O-rhamnoside, and 40,60-dihydroxy-4-methoxydihydrochalcone 20-O-?-D-glucopyranoside were isolated and identified by NMR analysis. The LC-MS/MS analysis led to the tentative identification of three novel dihydrochalcones namely 40,60-dihydroxy-4-methoxydihydrochalcone 20-O-rutinoside, 40,60-dihydroxy-4-methoxydihydrochalcone 20-O-glucosylpentoside and 40,60-dihydroxy-4-methoxydihydrochalcone 20-O-(3”-O-galloyl)-?-D-glucopyranoside. The structural identification of novel dihydrochalcones was based on the basic skeleton of the isolated dihydrochalcone, 40,60-dihydroxy-4-methoxydihydrochalcone 20-O-?-D-glucopyranoside and characteristic LC-MS/MS fragmentation patterns. This is the first comprehensive analysis for the identification of compounds from L. indica using LC-MS. A total 24 compounds including three new dihydrochalcones were identified for the first time from the genus Leea. © 2019 by the authors. | Source Title: | Molecules | URI: | https://scholarbank.nus.edu.sg/handle/10635/212326 | ISSN: | 14203049 | DOI: | 10.3390/molecules24040714 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_3390_molecules24040714.pdf | 1.68 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License