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Title: A MoS2-MWCNT based fluorometric nanosensor for exosome detection and quantification
Authors: Tayebi, M.
Tavakkoli Yaraki, M.
Yang, H.Y.
Ai, Y.
Issue Date: 2019
Publisher: Royal Society of Chemistry
Citation: Tayebi, M., Tavakkoli Yaraki, M., Yang, H.Y., Ai, Y. (2019). A MoS2-MWCNT based fluorometric nanosensor for exosome detection and quantification. Nanoscale Advances 1 (8) : 2866-2872. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial 4.0 International
Abstract: Circulating exosomes in body fluids are involved in many diseases and have important roles in pathophysiological processes. Specifically, they have emerged as a promising new class of biomarkers in cancer diagnosis and prognosis because of their high concentration and availability in a variety of biological fluids. The ability to quantitatively detect and characterize these nano-sized vesicles is crucial to make use of exosomes as a reliable biomarker for clinical applications. However, current methods are mostly technically challenging and time-consuming which prevents them from being adopted in clinical practice. In this work, we have developed a rapid sensitive platform for exosome detection and quantification by employing MoS2-multiwall carbon nanotubes as a fluorescence quenching material. This exosome biosensor shows a sensitive and selective biomarker detection. Using this MoS2-MWCNT based fluorometric nanosensor to analyze exosomes derived from MCF-7 breast cancer cells, we found that CD63 expression could be measured based on the retrieved fluorescence of the fluorophore with a good linear response range of 0-15% v/v. In addition, this nanosensing technique is able to quantify exosomes with different surface biomarker expressions and has revealed that exosomes secreted from MCF-7 breast cancer cells have a higher CD24 expression compared to CD63 and CD81. © 2019 The Royal Society of Chemistry.
Source Title: Nanoscale Advances
ISSN: 25160230
DOI: 10.1039/c9na00248k
Rights: Attribution-NonCommercial 4.0 International
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