Please use this identifier to cite or link to this item: https://doi.org/10.3390/v10010002
Title: Broadening the H5N3 vaccine immunogenicity against H5N1 virus by modification of neutralizing epitopes
Authors: Kumar, S.R. 
Chelvaretnam, S.
Tan, Y.
Prabakaran, M.
Keywords: Antibody-guided design
Epitope modified vaccine
Highly pathogenic avian influenza H5N1 (HPAI-H5N1)
Neutralizing epitopes
Issue Date: 2018
Publisher: MDPI AG
Citation: Kumar, S.R., Chelvaretnam, S., Tan, Y., Prabakaran, M. (2018). Broadening the H5N3 vaccine immunogenicity against H5N1 virus by modification of neutralizing epitopes. Viruses 10 (1) : 2. ScholarBank@NUS Repository. https://doi.org/10.3390/v10010002
Rights: Attribution 4.0 International
Abstract: The highly pathogenic avian influenza (HPAI) H5N1 virus remains to be one of the world’s largest pandemic threats due to the emergence of new variants. The rapid evolution of new sub-lineages is currently the greatest challenge in vaccine development. In this study, we developed an epitope modified non-pathogenic H5N3 (A/duck/Singapore/97) vaccine for broad protection against influenza H5 subtype. H5N3 hemagglutinin (HA) mutant reassortant viruses with A/Puerto Rico/8/34 (PR8) backbone were generated by mutating amino acids at the 140th loop and 190th ?-helix of hemagglutinin. The cross-neutralizing efficacy of reverse genetics-derived H5N3HA (RG-H5N3HA) mutants was confirmed by testing reactivity with reference chicken anti-H5N1 clade 2 virus sera. Furthermore, RG-H5N3HA mutant immunized mice induced cross-neutralizing antibodies and cross-protection against distinct H5N1 viral infection. Our findings suggest that the use of non-pathogenic H5 viruses antigenically related to HPAI-H5N1 allows for the development of broadly protective vaccines and reduces the need for biosafety level 3 (BSL3) containment facilities. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Viruses
URI: https://scholarbank.nus.edu.sg/handle/10635/210891
ISSN: 19994915
DOI: 10.3390/v10010002
Rights: Attribution 4.0 International
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