Please use this identifier to cite or link to this item: https://doi.org/10.1167/iovs.19-27566
Title: Three-dimensional neurite characterization of small incision lenticule extraction derived lenticules
Authors: Bandeira, F.
Yam, G.H.-F. 
Liu, Y.-C. 
Devarajan, K.
Mehta, J.S. 
Keywords: Corneal nerves
Corneal neurite function
Refractive surgery
SMILE
Stromal lenticules
Issue Date: 2019
Publisher: Association for Research in Vision and Ophthalmology Inc.
Citation: Bandeira, F., Yam, G.H.-F., Liu, Y.-C., Devarajan, K., Mehta, J.S. (2019). Three-dimensional neurite characterization of small incision lenticule extraction derived lenticules. Investigative Ophthalmology and Visual Science 60 (13) : 4408-4415. ScholarBank@NUS Repository. https://doi.org/10.1167/iovs.19-27566
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: PURPOSE. We study the density and excitatory response of neurites, and Schwann cells (SCs) in fresh and cryopreserved stromal lenticules derived from small incision lenticule extraction (SMILE). METHODS. Human stromal lenticules (n = 23) were immunostained for b III-tubulin and imaged using spinning disk confocal laser microscopy, followed by three-dimensional reconstruction, to reveal neurite distribution. The lenticule neurite density (LND) was assessed using a validated neurite tracing and length measurement method with NeuronJ. LND was compared among groups of different lenticule thickness (71–165 lm) obtained from -3 to >-6 diopters (D) corrections. SCs were identified by marker expression and the laser effect on SC-neurite interaction was examined under transmission electron microscopy (TEM). Fresh porcine SMILE-lenticules (n = 18) were used for LND comparison among storage conditions and functional excitatory calcium response assay. RESULTS. Using a validated neurite length measurement method, we found an inverse correlation of LND with lenticule thickness. Higher LND was found in thinner lenticules obtained from lower power of correction (r = -0.8925, P < 0.0001), whereas total lenticule neurite lengths did not alter significantly with regards to lenticule thickness. SCs were identified by GAP43 and p75NTR expression and were closely associated with lenticule neurites under TEM. In porcine lenticules, LND and excitatory calcium response were reduced after cold and cryogenic storage, when compared to fresh lenticules. CONCLUSIONS. The stromal neurites showed variations in density related to SMILE lenticule thickness and cryopreservation. With the presence of SC support and excitatory response, these neurite residues could retain minimal functionality that might serve as a potential advantage in the event of lenticule implantation. Copyright 2019 The Authors
Source Title: Investigative Ophthalmology and Visual Science
URI: https://scholarbank.nus.edu.sg/handle/10635/210736
ISSN: 01460404
DOI: 10.1167/iovs.19-27566
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1167_iovs_19-27566.pdf856.58 kBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

4
checked on May 14, 2022

Page view(s)

33
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons