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https://doi.org/10.1038/s41598-019-48688-y
Title: | Blo t 2: Group 2 allergen from the dust mite Blomia tropicalis | Authors: | Reginald, K. Pang, S.L. Chew, F.T. |
Issue Date: | 2019 | Publisher: | Nature Publishing Group | Citation: | Reginald, K., Pang, S.L., Chew, F.T. (2019). Blo t 2: Group 2 allergen from the dust mite Blomia tropicalis. Scientific Reports 9 (1) : 12239. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-019-48688-y | Rights: | Attribution 4.0 International | Abstract: | Blomia tropicalis has been recognized as a cause of allergic diseases in the tropical and subtropical regions. Here we report the immuno-characterization of its group 2 allergen, Blo t 2. Allergen Blo t 2 was amplified from the cDNA of B. tropicalis using degenerate primers, expressed in Escherichia coli as a recombinant protein and purified to homogeneity. The mature protein of Blo t 2 was 126 amino acids long with 52% sequence identity to Der p 2 and apparent molecular mass of 15 kDa. Circular dichroism spectroscopy showed that Blo t 2 is mainly a beta-sheeted protein. We confirmed the presence of three disulfide bonds in recombinant (r) Blo t 2 protein using electrospray mass spectrometry. Thirty-four percent of dust-mite allergic individuals from the Singapore showed specific IgE binding to rBlo t 2 as tested using immuno dot-blots. IgE-cross reactivity assays showed that Blo t 2 had between 20–50% of unique IgE-epitopes compared to Der p 2. IgE binding of native and recombinant forms of Blo t 2 were highly concordant (r2 = 0.77, p < 0.0001) to rBlo t 2. Dose-dependent in vitro histamine was observed when rBlo t 2 was incubated with whole blood of Blo t 2 sensitized individuals, demonstrating that it is a functional allergen. Nine naturally occurring isoforms of Blo t 2 were identified in this study, each having between 1–3 amino acid variations compared to the reference clone. Blo t 2 is a clinically relevant allergen of B. tropicalis as it has unique IgE epitopes compared to major group 2 allergens from Dermatophagoides spp. © 2019, The Author(s). | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/210687 | ISSN: | 20452322 | DOI: | 10.1038/s41598-019-48688-y | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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