Please use this identifier to cite or link to this item:
Title: Identification of cyclic dipeptides from Escherichia coli as new antimicrobial agents against Ralstonia solanacearum
Authors: Song, S.
Fu, S.
Sun, X.
Li, P.
Wu, J. 
Dong, T.
He, F.
Deng, Y.
Keywords: Antimicrobial activity
Bacterial wilt
Biocontrol agent
Cyclic dipeptides
Ralstonia solanacearum
Issue Date: 2018
Publisher: MDPI AG
Citation: Song, S., Fu, S., Sun, X., Li, P., Wu, J., Dong, T., He, F., Deng, Y. (2018). Identification of cyclic dipeptides from Escherichia coli as new antimicrobial agents against Ralstonia solanacearum. Molecules 23 (1) : 214. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Ralstonia solanacearum is a causative agent of bacterial wilt in many important crops throughout the world. How to control bacterial wilt caused by R. solanacearum is a major problem in agriculture. In this study, we aim to isolate the biocontrol agents that have high efficacy in the control of bacterial wilt. Three new bacterial strains with high antimicrobial activity against R. solanacearum GMI1000 were isolated and identified. Our results demonstrated that these bacteria could remarkably inhibit the disease index of host plant infected by R. solanacearum. It was indicated that strain GZ-34 (CCTCC No. M 2016353) showed an excellent protective effect to tomato under greenhouse conditions. Strain GZ-34 was characterized as Escherichia coli based on morphology, biochemistry, and 16S rRNA analysis. We identified that the main antimicrobial compounds produced by E. coli GZ-34 were cyclo(L-Pro-D-Ile) and cyclo(L-Pro-L-Phe) using electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) analysis. The two active compounds also interfered with the expression levels of some pathogenicity-contributors of R. solanacearum. Furthermore, cyclo(L-Pro-L-Phe) effectively inhibited spore formation of Magnaporthe grisea, which is a vital pathogenesis process of the fungal pathogen, suggesting cyclic dipeptides from E. coli are promising potential antimicrobial agents with broad-spectrum activity to kill pathogens or interfere with their pathogenesis. © 2018 by the authors.
Source Title: Molecules
ISSN: 1420-3049
DOI: 10.3390/molecules23010214
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_3390_molecules23010214.pdf2.74 MBAdobe PDF



Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons