Please use this identifier to cite or link to this item: https://doi.org/10.3390/s19112533
Title: Point-of-care surface plasmon resonance biosensor for stroke biomarkers NT-proBNP and s100? using a functionalized gold chip with specific antibody
Authors: Harpaz, D.
Koh, B.
Marks, R.S.
Seet, R.C.S. 
Abdulhalim, I.
Tok, A.I.Y.
Keywords: Au SPR-chip
Biomarkers
Biosensor
Diagnostics
NT-proBNP
Point-of-care
Refractive-index
S100?
Stroke
Surface-plasmon-resonance
Issue Date: 2019
Publisher: MDPI AG
Citation: Harpaz, D., Koh, B., Marks, R.S., Seet, R.C.S., Abdulhalim, I., Tok, A.I.Y. (2019). Point-of-care surface plasmon resonance biosensor for stroke biomarkers NT-proBNP and s100? using a functionalized gold chip with specific antibody. Sensors (Switzerland) 19 (11) : 2533. ScholarBank@NUS Repository. https://doi.org/10.3390/s19112533
Rights: Attribution 4.0 International
Abstract: Surface-plasmon-resonance (SPR) is a quantum-electromagnetic phenomenon arising from the interaction of light with free electrons at a metal-dielectric interface. At a specific angle/wavelength of light, the photon’s energy is transferred to excite the oscillation of the free electrons on the surface. A change in the refractive-index (RI) may occur, which is influenced by the analyte concentration in the medium in close contact with the metal surface. SPR has been widely used for the detection of gaseous, liquid, or solid samples. In this study, a functionalized specific SPR chip was designed and used in a novel point-of-care SPR module (PhotonicSys SPR H5) for the detection of the stroke biomarkers NT-proBNP and S100?. These biomarkers have proven to be good for stroke diagnosis, with sensitivity and specificity of >85%. Specific detection was done by binding a biomolecular-recognizing antibody onto the Au SPR-chip. Detection was tested in water and plasma samples. NT-proBNP and S100? were detected in a range of concentrations for stroke, from 0.1 ng/mL to 10 ng/mL. The RI of the blank plasma samples was 1.362412, and the lowest concentration tested for both biomarkers showed a prominent shift in the RI signal (0.25 ng/mL NT-proBNP (1.364215) and S100? (1.364024)). The sensor demonstrated a clinically relevant limit-of-detection of less than ng/mL. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Sensors (Switzerland)
URI: https://scholarbank.nus.edu.sg/handle/10635/209573
ISSN: 1424-8220
DOI: 10.3390/s19112533
Rights: Attribution 4.0 International
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