Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.stemcr.2019.08.005
Title: A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State
Authors: Szczerbinska, I.
Gonzales, K.A.U.
Cukuroglu, E.
Ramli, M.N.B.
Lee, B.P.G.
Tan, C.P.
Wong, C.K.
Rancati, G.I.
Liang, H.
Göke, J.
Ng, H.-H. 
Chan, Y.-S.
Keywords: AZD5438
BCR-ABL and SRC inhibitor
CDK1/2/9 inhibitor
dasatinib
endogenous retroviral element
feeder-independent and chemically defined culture
naive human pluripotent cell state
small-molecule screens
Issue Date: 2019
Publisher: Cell Press
Citation: Szczerbinska, I., Gonzales, K.A.U., Cukuroglu, E., Ramli, M.N.B., Lee, B.P.G., Tan, C.P., Wong, C.K., Rancati, G.I., Liang, H., Göke, J., Ng, H.-H., Chan, Y.-S. (2019). A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State. Stem Cell Reports 13 (4) : 612-626. ScholarBank@NUS Repository. https://doi.org/10.1016/j.stemcr.2019.08.005
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols relying on extraneous undefined factors such as feeders. Here we performed a small-molecule screen to identify compounds that facilitate chemically defined establishment and maintenance of human feeder-independent naive embryonic (FINE) stem cells. The expression profile in genic and repetitive elements of FINE cells resembles the 8-cell-to-morula stage in vivo, and only differs from feeder-dependent naive cells in genes involved in cell-cell/cell-matrix interactions. FINE cells offer several technical advantages, such as increased amenability to transfection and a longer period of genomic stability, compared with feeder-dependent cells. Thus, FINE cells will serve as an accessible and useful system for scientific and translational applications of naïve pluripotent stem cells. © 2019 The Author(s)In this article, Chan and colleagues develop a chemically defined culture system for the establishment and maintenance of human feeder-independent naive embryonic (FINE) stem cells. FINE cells offer several technical advantages beyond feeder independence such as enhanced genomic stability, increased amenability to transfection, and expression of 8-cell-to-morula stage markers. © 2019 The Author(s)
Source Title: Stem Cell Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/209541
ISSN: 2213-6711
DOI: 10.1016/j.stemcr.2019.08.005
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1016_j_stemcr_2019_08_005.pdf5.21 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

14
checked on Feb 2, 2023

Page view(s)

87
checked on Feb 2, 2023

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons