Please use this identifier to cite or link to this item:
Title: Epstein-Barr virus-associated primary nodal T/NK-cell lymphoma shows a distinct molecular signature and copy number changes
Authors: Ng, Siok-Bian 
Chung, Tae-Hoon
Kato, Seiichi
Nakamura, Shigeo
Takahashi, Emiko
Ko, Young-Hyeh
Khoury, Joseph D
Yin, C Cameron
Soong, Richie
Jeyasekharan, Anand D 
Hoppe, Michal Marek
Selvarajan, Viknesvaran
Tan, Soo-Yong
Lim, Soon-Thye
Ong, Choon-Kiat 
Nairismagi, Maarja-Liisa
Maheshwari, Priyanka 
Choo, Shoa-Nian 
Fan, Shuangyi 
Lee, Chi-Kuen 
Chuang, Shih-Sung
Chng, Wee-Joo 
Keywords: Science & Technology
Life Sciences & Biomedicine
Issue Date: 1-Feb-2018
Citation: Ng, Siok-Bian, Chung, Tae-Hoon, Kato, Seiichi, Nakamura, Shigeo, Takahashi, Emiko, Ko, Young-Hyeh, Khoury, Joseph D, Yin, C Cameron, Soong, Richie, Jeyasekharan, Anand D, Hoppe, Michal Marek, Selvarajan, Viknesvaran, Tan, Soo-Yong, Lim, Soon-Thye, Ong, Choon-Kiat, Nairismagi, Maarja-Liisa, Maheshwari, Priyanka, Choo, Shoa-Nian, Fan, Shuangyi, Lee, Chi-Kuen, Chuang, Shih-Sung, Chng, Wee-Joo (2018-02-01). Epstein-Barr virus-associated primary nodal T/NK-cell lymphoma shows a distinct molecular signature and copy number changes. HAEMATOLOGICA 103 (2) : 278-287. ScholarBank@NUS Repository.
Abstract: The molecular biology of primary nodal T- and NK-cell lymphoma and its relationship with extranodal NK/T-cell lymphoma, nasal type is poorly understood. In this study, we assessed the relationship between nodal and extranodal Epstein-Barr virus-positive T/NK-cell lymphomas using gene expression profiling and copy number aberration analyses. We performed gene expression profiling and copy number aberration analysis on 66 cases of Epstein-Barr virus-associated T/NK-cell lymphoma from nodal and extranodal sites, and correlated the molecular signatures with clinicopathological features. Three distinct molecular clusters were identified with one enriched for nodal presentation and loss of 14q11.2 (TCRA loci). T/NK-cell lymphomas with a nodal presentation (nodal-group) were significantly associated with older age, lack of nasal involvement, and T-cell lineage compared to those with an extranodal presentation (extranodal-group). On multivariate analysis, nodal presentation was an independent factor associated with short survival. Comparing the molecular signatures of the nodal and extranodal groups it was seen that the former was characterized by upregulation of PD-L1 and T-cell-related genes, including CD2 and CD8, and downregulation of CD56, consistent with the CD8+/CD56-immunophenotype. PD-L1 and CD2 protein expression levels were validated using multiplexed immunofluorescence. Interestingly, nodal group lymphomas were associated with 14q11.2 loss which correlated with loss of TCR loci and T-cell origin. Overall, our results suggest that T/NK-cell lymphoma with nodal presentation is distinct and deserves to be classified separately from T/NK-cell lymphoma with extranodal presentation. Upregulation of PD-L1 indicates that it may be possible to use anti-PD1 immunotherapy in this distinctive entity. In addition, loss of 14q11.2 may be a potentially useful diagnostic marker of T-cell lineage.
ISSN: 03906078
DOI: 10.3324/haematol.2017.180430
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Ng SB. Haematologica 2018. EBV primary nodal TNK lymphoma.pdfPublished version4.53 MBAdobe PDF



Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.