Functional analysis of circular RNAs
Shanmugapriya Huda, HA Vijayarathna, S Oon, CE Chen, Y Kanwar, JR Ng, ML Sasidharan, S
Shanmugapriya
Huda, HA
Vijayarathna, S
Oon, CE
Chen, Y
Kanwar, JR
Sasidharan, S
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Abstract
Circular RNAs characterize a class of widespread and diverse endogenous RNAs which are non-coding RNAs that are made by back-splicing events and have covalently closed loops with no polyadenylated tails. Various indications specify that circular RNAs (circRNAs) are plentiful in the human transcriptome. However, their participation in biological processes remains mostly undescribed. To date thousands of circRNAs have been revealed in organisms ranging from Drosophila melanogaster to Homo sapiens. Functional studies specify that these transcripts control expression of protein-coding linear transcripts and thus encompass a key component of gene expression regulation. This chapter provide a comprehensive overview on functional validation of circRNAs. Furthermore, we discuss the recent modern methodologies for the functional validation of circRNAs such as RNA interference (RNAi) gene silencing assay, luciferase reporter assays, circRNA gain-of-function investigation via overexpression of circular transcript assay, RT-q-PCR quantification, and other latest applicable assays. The methods described in this chapter are demonstrated on the cellular model.
Keywords
Cellular model, CircRNAs, Functional validation, Alternative Splicing, Animals, Computational Biology, Databases, Genetic, Gene Expression Regulation, Genes, Reporter, Humans, In Situ Hybridization, RNA, RNA, Circular, RNA, Long Noncoding, Reverse Transcriptase Polymerase Chain Reaction, Software
Source Title
Advances in Experimental Medicine and Biology
Publisher
Springer Singapore
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Date
2018-01-01
DOI
10.1007/978-981-13-1426-1_8
Type
Book Chapter