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https://doi.org/10.1093/nar/gkx1105
Title: | C/EBPβ mediates RNA polymerase III-driven transcription of oncomiR-138 in malignant gliomas | Authors: | Di Pascale, F. Nama, S. Muhuri, M. Quah, S. Ismail, H.M. Chan, X.H.D. Sundaram, G.M. Ramalingam, R. Burke, B. Sampath, P. |
Issue Date: | 2018 | Publisher: | Oxford University Press | Citation: | Di Pascale, F., Nama, S., Muhuri, M., Quah, S., Ismail, H.M., Chan, X.H.D., Sundaram, G.M., Ramalingam, R., Burke, B., Sampath, P. (2018). C/EBPβ mediates RNA polymerase III-driven transcription of oncomiR-138 in malignant gliomas. Nucleic Acids Research 46 (1) : 336-349. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkx1105 | Rights: | Attribution-NonCommercial 4.0 International | Abstract: | MicroRNA-138 (miR-138) is a pro-survival oncomiR for glioma stem cells. In malignant gliomas, dysregulated expression of microRNAs, such as miR-138, promotes Tumour initiation and progression. Here, we identify the ancillary role of the CCAAT/enhancer binding protein ? (C/EBP?) as a transcriptional activator of miR-138. We demonstrate that a short 158 bp DNA sequence encoding the precursor of miR-138-2 is essential and sufficient for transcription of miR-138. This short sequence includes the A-box and B-box elements characteristic of RNA Polymerase III (Pol III) promoters, and is also directly bound by C/EBP? via an embedded 'C/EBP? responsive element' (CRE). CRE and the Pol III B-box element overlap, suggesting that C/EBP? and transcription factor 3C (TFIIIC) interact at the miR-138-2 locus. We propose that this interaction is essential for the recruitment of the RNA Pol III initiation complex and associated transcription of the oncomiR, miR-138 in malignant gliomas. © The Author(s) 2017. | Source Title: | Nucleic Acids Research | URI: | https://scholarbank.nus.edu.sg/handle/10635/206470 | ISSN: | 03051048 | DOI: | 10.1093/nar/gkx1105 | Rights: | Attribution-NonCommercial 4.0 International |
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