Please use this identifier to cite or link to this item: https://doi.org/10.1093/nar/gkx1105
Title: C/EBPβ mediates RNA polymerase III-driven transcription of oncomiR-138 in malignant gliomas
Authors: Di Pascale, F.
Nama, S.
Muhuri, M.
Quah, S.
Ismail, H.M.
Chan, X.H.D.
Sundaram, G.M.
Ramalingam, R.
Burke, B.
Sampath, P. 
Issue Date: 2018
Publisher: Oxford University Press
Citation: Di Pascale, F., Nama, S., Muhuri, M., Quah, S., Ismail, H.M., Chan, X.H.D., Sundaram, G.M., Ramalingam, R., Burke, B., Sampath, P. (2018). C/EBPβ mediates RNA polymerase III-driven transcription of oncomiR-138 in malignant gliomas. Nucleic Acids Research 46 (1) : 336-349. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkx1105
Rights: Attribution-NonCommercial 4.0 International
Abstract: MicroRNA-138 (miR-138) is a pro-survival oncomiR for glioma stem cells. In malignant gliomas, dysregulated expression of microRNAs, such as miR-138, promotes Tumour initiation and progression. Here, we identify the ancillary role of the CCAAT/enhancer binding protein ? (C/EBP?) as a transcriptional activator of miR-138. We demonstrate that a short 158 bp DNA sequence encoding the precursor of miR-138-2 is essential and sufficient for transcription of miR-138. This short sequence includes the A-box and B-box elements characteristic of RNA Polymerase III (Pol III) promoters, and is also directly bound by C/EBP? via an embedded 'C/EBP? responsive element' (CRE). CRE and the Pol III B-box element overlap, suggesting that C/EBP? and transcription factor 3C (TFIIIC) interact at the miR-138-2 locus. We propose that this interaction is essential for the recruitment of the RNA Pol III initiation complex and associated transcription of the oncomiR, miR-138 in malignant gliomas. © The Author(s) 2017.
Source Title: Nucleic Acids Research
URI: https://scholarbank.nus.edu.sg/handle/10635/206470
ISSN: 03051048
DOI: 10.1093/nar/gkx1105
Rights: Attribution-NonCommercial 4.0 International
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