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https://doi.org/10.1038/s41598-019-42162-5
Title: | Silencing an insulin-induced lncRNA, LncASIR, impairs the transcriptional response to insulin signalling in adipocytes | Authors: | Degirmenci, U. Li, J. Lim, Y.C. Siang, D.T.C. Lin, S. Liang, H. Sun, L. |
Issue Date: | 2019 | Publisher: | Nature Publishing Group | Citation: | Degirmenci, U., Li, J., Lim, Y.C., Siang, D.T.C., Lin, S., Liang, H., Sun, L. (2019). Silencing an insulin-induced lncRNA, LncASIR, impairs the transcriptional response to insulin signalling in adipocytes. Scientific Reports 9 (1) : 5608. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-019-42162-5 | Rights: | Attribution 4.0 International | Abstract: | Long noncoding RNA(lncRNA)s are new regulators governing the metabolism in adipose tissue. In this study, we aimed to understand how lncRNAs respond to insulin signalling and explore whether lncRNAs have a functional role in insulin signalling pathway. We treated primary adipocyte cultures with insulin and collected RNA for RNA-sequencing to profile the non-coding transcriptome changes, through which we identified a top Adipose Specific Insulin Responsive LncRNA (LncASIR). To determine its biological function, we knocked down LncASIR using dcas9-KRAB, followed by RNA-seq to examine the effect on insulin-induced gene expression program. We identified a set of lncRNAs regulated by insulin signalling pathway. LncASIR is transcribed from a super enhancer region and responds robustly to insulin treatment. Silencing LncASIR resulted in an impaired global insulin-responsive gene program. LncASIR is a novel and integral component in the insulin signalling pathway in adipocytes. © 2019, The Author(s). | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/206252 | ISSN: | 2045-2322 | DOI: | 10.1038/s41598-019-42162-5 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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