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https://doi.org/10.1186/s13104-019-4730-1
Title: | The analysis of DMD gene deletions by multiplex PCR in Indonesian DMD/BMD patients: The era of personalized medicine | Authors: | Iskandar, K Dwianingsih, EK Pratiwi, L Kalim, AS Mardhiah, H Putranti, AH Nurputra, DK Triono, A Herini, ES Malueka, RG Gunadi Lai, PS Sunartini |
Keywords: | DMD gene deletion Duchenne/Becker muscular dystrophy Exon skipping therapy Multiplex PCR Adolescent Child Child, Preschool Dystrophin Exons Gene Deletion Humans Indonesia Infant Male Multiplex Polymerase Chain Reaction Muscular Dystrophy, Duchenne Precision Medicine |
Issue Date: | 28-Oct-2019 | Publisher: | Springer Science and Business Media LLC | Citation: | Iskandar, K, Dwianingsih, EK, Pratiwi, L, Kalim, AS, Mardhiah, H, Putranti, AH, Nurputra, DK, Triono, A, Herini, ES, Malueka, RG, Gunadi, Lai, PS, Sunartini (2019-10-28). The analysis of DMD gene deletions by multiplex PCR in Indonesian DMD/BMD patients: The era of personalized medicine. BMC Research Notes 12 (1) : 704-. ScholarBank@NUS Repository. https://doi.org/10.1186/s13104-019-4730-1 | Abstract: | Objective: Duchenne/Becker muscular dystrophy (DMD/BMD) is the most common genetic neuromuscular disease in children, resulting from a defect in the DMD gene located on Xp21.2. The new emerging treatment using exon skipping strategy is tailored to specific mutations, thus molecular diagnostics are particularly important. This study aimed to detect the DMD gene deletion in Indonesian DMD/BMD patients and analyze the potential amenability by exon skipping therapy. Results: Thirty-four male patients were enrolled in this study, 23 of them (67.6%) underwent muscle biopsy and showed the absence or partially expressed dystrophin protein in immunohistochemistry staining. All patients had very high serum CK levels (10.529 ± 9.97 IU/L). Multiplex PCR revealed the DMD gene deletions in 15 (44.1%) cases. Seventy-eight percent of deletions were clustered in the hot-spot region of exon 43 to 52. Furthermore, seven (20.5%) patients were potentially amenable to exon skipping treatment. Therefore, multiplex PCR is one feasible method to detect DMD gene deletion in Indonesian DMD/BMD patients that can further determine the potential amenability of exon skipping therapy. In addition, this study is the first report of DMD gene deletion analysis in Indonesia. | Source Title: | BMC Research Notes | URI: | https://scholarbank.nus.edu.sg/handle/10635/206168 | ISSN: | 17560500 | DOI: | 10.1186/s13104-019-4730-1 |
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