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Title: Electrospun fibers enhanced the paracrine signaling of mesenchymal stem cells for cartilage regeneration
Authors: Kadir, ND 
Yang, Z 
Hassan, A 
Denslin, V 
Lee, EH 
Keywords: Cartilage repair
Fiber orientation
Mechanotransduction pathway
Mesenchymal stem cells
Paracrine signaling
Cell Differentiation
Mechanotransduction, Cellular
Mesenchymal Stem Cells
Paracrine Communication
Issue Date: 1-Dec-2021
Publisher: Springer Science and Business Media LLC
Citation: Kadir, ND, Yang, Z, Hassan, A, Denslin, V, Lee, EH (2021-12-01). Electrospun fibers enhanced the paracrine signaling of mesenchymal stem cells for cartilage regeneration. Stem Cell Research and Therapy 12 (1) : 100-. ScholarBank@NUS Repository.
Abstract: Background: Secretome profiles of mesenchymal stem cells (MSCs) are reflective of their local microenvironments. These biologically active factors exert an impact on the surrounding cells, eliciting regenerative responses that create an opportunity for exploiting MSCs towards a cell-free therapy for cartilage regeneration. The conventional method of culturing MSCs on a tissue culture plate (TCP) does not provide the physiological microenvironment for optimum secretome production. In this study, we explored the potential of electrospun fiber sheets with specific orientation in influencing the MSC secretome production and its therapeutic value in repairing cartilage. Methods: Conditioned media (CM) were generated from MSCs cultured either on TCP or electrospun fiber sheets of distinct aligned or random fiber orientation. The paracrine potential of CM in affecting chondrogenic differentiation, migration, proliferation, inflammatory modulation, and survival of MSCs and chondrocytes was assessed. The involvement of FAK and ERK mechanotransduction pathways in modulating MSC secretome were also investigated. Results: We showed that conditioned media of MSCs cultured on electrospun fiber sheets compared to that generated from TCP have improved secretome yield and profile, which enhanced the migration and proliferation of MSCs and chondrocytes, promoted MSC chondrogenesis, mitigated inflammation in both MSCs and chondrocytes, as well as protected chondrocytes from apoptosis. Amongst the fiber sheet-generated CM, aligned fiber-generated CM (ACM) was better at promoting cell proliferation and augmenting MSC chondrogenesis, while randomly oriented fiber-generated CM (RCM) was more efficient in mitigating the inflammation assault. FAK and ERK signalings were shown to participate in the modulation of MSC morphology and its secretome production. Conclusions: This study demonstrates topographical-dependent MSC paracrine activities and the potential of employing electrospun fiber sheets to improve the MSC secretome for cartilage regeneration.
Source Title: Stem Cell Research and Therapy
ISSN: 17576512
DOI: 10.1186/s13287-021-02137-8
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