Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M111.268573
Title: MicroRNA-processing Enzyme Dicer Is Required in Epicardium for Coronary Vasculature Development
Authors: Singh, Manvendra K 
Lu, Min Min 
Massera, Daniele
Epstein, Jonathan A
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
EPITHELIAL-MESENCHYMAL TRANSITIONS
NEURAL CREST
PROGENITOR CELLS
MOUSE DEVELOPMENT
ARTERY FORMATION
CHICK-EMBRYOS
HEART
MUSCLE
DIFFERENTIATION
CARDIOMYOCYTE
Issue Date: 25-Nov-2011
Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation: Singh, Manvendra K, Lu, Min Min, Massera, Daniele, Epstein, Jonathan A (2011-11-25). MicroRNA-processing Enzyme Dicer Is Required in Epicardium for Coronary Vasculature Development. JOURNAL OF BIOLOGICAL CHEMISTRY 286 (47) : 41036-41045. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M111.268573
Abstract: The epicardium is a sheet of epithelial cells covering the heart during early cardiac development. In recent years, the epicardium has been identified as an important contributor to cardiovascular development, and epicardium-derived cells have the potential to differentiate into multiple cardiac cell lineages. Some epicardium-derived cells that undergo epithelial-to-mesenchymal transition and delaminate from the surface of the developing heart subsequently invade the myocardium and differentiate into vascular smooth muscle of the developing coronary vasculature. MicroRNAs (miRNAs) have been implicated broadly in tissue patterning and development, including in the heart, but a role in epicardium is unknown. To examine the role of miRNAs during epicardial development, we conditionally deleted the miRNA-processing enzyme Dicer in the proepicardium using Gata5-Cre mice. Epicardial Dicer mutant mice are born in expected Mendelian ratios but die immediately after birth with profound cardiac defects, including impaired coronary vessel development. We found that loss of Dicer leads to impaired epicardial epithelial-to-mesenchymal transition and a reduction in epicardial cell proliferation and differentiation into coronary smooth muscle cells. These results demonstrate a critical role for Dicer, and by implication miRNAs, in murine epicardial development. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
Source Title: JOURNAL OF BIOLOGICAL CHEMISTRY
URI: https://scholarbank.nus.edu.sg/handle/10635/201369
ISSN: 00219258
1083351X
DOI: 10.1074/jbc.M111.268573
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