Please use this identifier to cite or link to this item: https://doi.org/10.1126/sciadv.aba1190
Title: Diversification of reprogramming trajectories revealed by parallel single-cell transcriptome and chromatin accessibility sequencing
Authors: Xing, Q.R.
El Farran, C.A.
Gautam, P.
Chuah, Y.S.
Warrier, T.
Toh, C.X.D.
Kang, N.Y.
Sugii, S.
Chang, Y.T. 
Xu, J. 
Collins, J.J.
Daley, G.Q.
Li, H.
Zhang, L.F.
Loh, Y.H. 
Issue Date: 2020
Publisher: American Association for the Advancement of Science
Citation: Xing, Q.R., El Farran, C.A., Gautam, P., Chuah, Y.S., Warrier, T., Toh, C.X.D., Kang, N.Y., Sugii, S., Chang, Y.T., Xu, J., Collins, J.J., Daley, G.Q., Li, H., Zhang, L.F., Loh, Y.H. (2020). Diversification of reprogramming trajectories revealed by parallel single-cell transcriptome and chromatin accessibility sequencing. Science Advances 6 (37) : eaba1190. ScholarBank@NUS Repository. https://doi.org/10.1126/sciadv.aba1190
Rights: Attribution-NonCommercial 4.0 International
Abstract: Cellular reprogramming suffers from low efficiency especially for the human cells. To deconstruct the heterogeneity and unravel the mechanisms for successful reprogramming, we adopted single-cell RNA sequencing (scRNA-Seq) and single-cell assay for transposase-accessible chromatin (scATAC-Seq) to profile reprogramming cells across various time points. Our analysis revealed that reprogramming cells proceed in an asynchronous trajectory and diversify into heterogeneous subpopulations. We identified fluorescent probes and surface markers to enrich for the early reprogrammed human cells. Furthermore, combinatory usage of the surface markers enabled the fine segregation of the early-intermediate cells with diverse reprogramming propensities. scATAC-Seq analysis further uncovered the genomic partitions and transcription factors responsible for the regulatory phasing of reprogramming process. Binary choice between a FOSL1 and a TEAD4-centric regulatory network determines the outcome of a successful reprogramming. Together, our study illuminates the multitude of diverse routes transversed by individual reprogramming cells and presents an integrative roadmap for identifying the mechanistic part list of the reprogramming machinery. Copyright © 2020 The Authors, some rights reserved.
Source Title: Science Advances
URI: https://scholarbank.nus.edu.sg/handle/10635/197555
ISSN: 23752548
DOI: 10.1126/sciadv.aba1190
Rights: Attribution-NonCommercial 4.0 International
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1126_sciadv_aba1190.pdf3.72 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

12
checked on Sep 22, 2022

Page view(s)

93
checked on Sep 22, 2022

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons